Human Clusterin ELISA Kit
Cat no : KE00110
Synonyms
AAG4, Apo J, APOJ, ApoJalpha, ApoJbeta, Apolipoprotein J, CLI, CLU, clusterin, Ku70 binding protein 1, KUB1, NA1/NA2, SGP 2, SGP2, SP 40, TRPM 2, TRPM2
Validation Data Gallery
Product Information
KE00110 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Clusterin ELISA kit is to be used to detect and quantify protein levels of endogenous Clusterin. The assay recognizes human Clusterin. An antibody specific for Clusterin has been pre-coated onto the microwells. The Clusterin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human Clusterin is added to detect the captured human Clusterin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Clusterin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Urine, Saliva |
| Assay type | Sandwich |
| Sensitivity | 5.0 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1191 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 102% | 91%-118% |
| Cell culture supernatants | 96% | 72%-121% |
| Urine | 99% | 85%-123% |
| Saliva | 93% | 80%-112% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,832.2 | 52.3 | 2.9 |
| 2 | 20 | 451.4 | 12.3 | 2.7 |
| 3 | 20 | 109.4 | 5.0 | 4.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,771.9 | 96.8 | 5.5 |
| 2 | 24 | 427.4 | 22.9 | 5.3 |
| 3 | 24 | 95.8 | 8.2 | 8.5 |
Background Information
Clusterin (also known as apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids. It is encoded by one single gene and cleaved posttranslationally into α and β subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, regulating complement. Increased plasma clusterin levels have been associated with severity, pathology, and progression in Alzheimer disease.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AAG4, Apo J, APOJ, ApoJalpha, ApoJbeta, Apolipoprotein J, CLI, CLU, clusterin, Ku70 binding protein 1, KUB1, NA1/NA2, SGP 2, SGP2, SP 40, TRPM 2, TRPM2 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Life Sci Alliance C9ORF72-derived poly-GA DPRs undergo endocytic uptake in iAstrocytes and spread to motor neurons. |