Human Clusterin ELISA Kit
Cat no : KE00110
Synonyms
AAG4, Aging-associated gene 4 protein, Apo J, APOJ, Apo-J
Validation Data Gallery
Product Information
KE00110 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Clusterin ELISA kit is to be used to detect and quantify protein levels of endogenous Clusterin. The assay recognizes human Clusterin. An antibody specific for Clusterin has been pre-coated onto the microwells. The Clusterin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human Clusterin is added to detect the captured human Clusterin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Clusterin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Urine, Saliva |
| Assay type | Sandwich |
| Sensitivity | 5.0 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1191 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 102% | 91%-118% |
| Cell culture supernatants | 96% | 72%-121% |
| Urine | 99% | 85%-123% |
| Saliva | 93% | 80%-112% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,832.2 | 52.3 | 2.9 |
| 2 | 20 | 451.4 | 12.3 | 2.7 |
| 3 | 20 | 109.4 | 5.0 | 4.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,771.9 | 96.8 | 5.5 |
| 2 | 24 | 427.4 | 22.9 | 5.3 |
| 3 | 24 | 95.8 | 8.2 | 8.5 |
Background Information
Clusterin (also known as apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biological fluids. It is encoded by one single gene and cleaved posttranslationally into α and β subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, and regulating complement. Increased plasma clusterin levels have been associated with the severity, pathology, and progression of Alzheimer's disease.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | AAG4, Aging-associated gene 4 protein, Apo J, APOJ, Apo-J |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Clin Chim Acta Clinical implications of serum adropin and clusterin in chronic renal failure patients who received hemodialysis | ||
Life Sci Alliance C9ORF72-derived poly-GA DPRs undergo endocytic uptake in iAstrocytes and spread to motor neurons. |