Human FKBPL ELISA Kit
Cat no : KE00023
Synonyms
DIR1, FK506 binding protein like, FKBPL, NG7, WISP39
Validation Data Gallery
Product Information
KE00023 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The FKBPL ELISA kit is to be used to detect and quantify protein levels of endogenous FKBPL. The assay recognizes human FKBPL. An antibody specific for FKBPL has been pre-coated onto the microwells. The FKBPL protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for FKBPL is added to detect the captured FKBPL protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human FKBPL ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 7.0 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 63943 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 99% | 87%-115% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,084.9 | 63.2 | 5.8 |
| 2 | 20 | 266.4 | 15.5 | 5.8 |
| 3 | 20 | 80.8 | 7.8 | 9.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,112.7 | 55.6 | 5.0 |
| 2 | 24 | 275.0 | 26.7 | 9.7 |
| 3 | 24 | 85.8 | 4.7 | 5.4 |
Background Information
FKBPL, also named as DIR1, NG7 and WISp39, has similarity to the immunophilin protein family, which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. It is thought to have a potential role in the induced radioresistance, probably by increasing the rate of DNA repair in cells exposed to X rays. Breast cancer cells stably overexpressing FKBPL became dependent on estrogen for their growth and were dramaticaly more sensitive to the antiestrogens Tamoxifen and Fulvestrant, whereas FKBPL knockdown reverses this phenotype. FKBPL is an estrogen-inducible gene that acts as a cochaperone in ERa/Hsp90 molecular complexes; furthermore, FKBPL levels may be both a prognostic indicator and determinant of response to endocrine therapy. This kit is used to quantify FKBPL level in vivo.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | DIR1, FK506 binding protein like, FKBPL, NG7, WISP39 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.