Recombinant Human GITR/TNFRSF18 protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg1356

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Validation Data Gallery

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Product Information

• Purity: >90 %, SDS-PAGE
• Endotoxin: <1.0 EU/μg protein, LAL method
• Activity:

  Not tested

• Expression: HEK293-derived Human GITR/TNFRSF18 protein Gln26-Glu161 (Accession# Q9Y5U5-1) with a rabbit IgG Fc tag at the C-terminus.
• GeneID: 8784
• Accession: Q9Y5U5-1
• PredictedSize: 40.8 kDa
• Formulation: Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Reconstitution: Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
• Storage Conditions:
  It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

GITR is a type I transmembrane protein that contains a cysteine-rich extracellular domain and an intracellular domain that is highly homologous to TNFR family members 4-1BB/CD137 and CD27. GITR is expressed on a variety of immune cells, including regulatory T cells (Tregs), effector T cells (Teffs), natural killer cells (NK cells), B cells, and certain myeloid cells. Activation of GITR can promote the activation, proliferation, and cytokine secretion of effector T cells.

References:

1. Oja, Anna E et al. Immunology letters vol. 222 (2020): 73-79.
2. Wang, Feng et al. Nature communications vol. 12,1 (2021): 1378.
3. Ming, Siqi et al. Frontiers in immunology vol. 12 (2021): 736269.
4. Chan, Sarah et al. Nature cancer vol. 3,3 (2022): 337-354.