Human GKN1 ELISA Kit

Catalog number: KE00029

$599.00

Freight/Packing £00.00

Size: 09µ/gram

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Overview


Product name:
Human GKN1 ELISA Kit

Tests:
1 X 96 well plate

Sample type:
serum; Plasma

Assay type:
Sandwich

Sensitivity:
5 pg/mL

Range:
31.25-2000 pg/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Plasma 95% 85%-121%

Product overview:
KE00029 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The GKN1 ELISA kit is to be used to detect and quantify protein levels of endogenous GKN1. The assay recognizes human GKN1. A polyclonal antibody specific for GKN1 has been pre-coated onto the microwells. The GKN1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for GKN1 is added to detect the captured GKN1 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
18 kDa antrum mucosa protein, AMP 18, AMP18, BRICD1, CA11, FOV, foveolin, gastrokine 1, GKN1, Protein CA11
Background

Gastrokine 1 (GKN1), a stomach-speci?c protein also known as 18 kDa antrum mucosa protein (AMP-18) or foveolin, belongs to the gastrokine family of gastric mucus cell-secreted proteins. The human GKN1 gene is localized in a region of chromosome 2p13 of about 6 kb and contains 6 exons. As a secreted protein, GKN1 is specifically expressed in gastric mucosa to protect and maintain the integrity of gastric epithelium. GKN1 is involved in the replenishment of the surface lumen epithelial cell layer, and could play a role in cell proliferation and differentiation. Recent findings showed that GKN1 may act as a functional gastric tumor suppressor and may also modulate apoptotic signals.


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