Human GM-CSF ELISA Kit
Cat no : KE00003
Synonyms
Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, Molgramostin, Sargramostim
Validation Data Gallery
Product Information
KE00003 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The GM-CSF ELISA kit is to be used to detect and quantify protein levels of endogenous GM-CSF. The assay recognizes human GM-CSF. An antibody specific for GM-CSF has been pre-coated onto the microwells. The GM-CSF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for GM-CSF is added to detect the captured GM-CSF protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human GM-CSF ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 1.0 pg/mL |
| Range | 3.9-250 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1437 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plamsa | 93% | 80%-109% |
| Cell culture supernatants | 92% | 77%-107% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 206.1 | 12.1 | 5.9 |
| 2 | 20 | 40.7 | 2.8 | 6.9 |
| 3 | 20 | 5.3 | 0.4 | 7.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 272.8 | 20.9 | 7.7 |
| 2 | 24 | 72.3 | 4.1 | 5.7 |
| 3 | 24 | 11.8 | 1.0 | 8.8 |
Background Information
CSF2, also named as GM-CSF, is an important hematopoietic growth factor and immune modulator, which is produced by a variety of cell types including T cells, macrophages, endothelial cells and fibroblasts upon receiving immune stimuli. It was originally recognized as a stimulator for the proliferation of granulocytes and macrophages from bone marrow precursor cells. It has also been shown to promote the survival and activation of mature myeloid cells and therefore contributes to the maintenance of innate immune homeostasis. Recent studies suggest that GM-CSF also has proinflammatory functions and plays critical roles in the development of autoimmune and inflammatory diseases, particularly in Th17 driven diseases. GM-CSF also plays a role in embryonic development by functioning as an embryokine produced by reproductive tract.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, Molgramostin, Sargramostim |
Publications
| Species | Sample Type | Title |
|---|---|---|
Aging (Albany NY) Relationship between senescence in macaques and bone marrow mesenchymal stem cells and the molecular mechanism. | ||
J Clin Invest IL20RB mediates tumoral response to osteoclastic niches and promotes bone metastasis of lung cancer | ||
Mol Ther Nucleic Acids Intratumoral delivered novel circular mRNA encoding cytokines for immune modulation and cancer therapy | ||
Cancer Impact of tocilizumab on anti-CD19 chimeric antigen receptor T-cell therapy in B-cell acute lymphoblastic leukemia |