Human GM-CSF ELISA Kit

Sensitivity

1.0 pg/mL

Range

3.9-250 pg/mL

Reacted Species

Human

Cat no : KE00003

Synonyms

Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, Molgramostin, Sargramostim



Product Information

KE00003 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The GM-CSF ELISA kit is to be used to detect and quantify protein levels of endogenous GM-CSF. The assay recognizes human GM-CSF. An antibody specific for GM-CSF has been pre-coated onto the microwells. The GM-CSF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for GM-CSF is added to detect the captured GM-CSF protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Product name Human GM-CSF ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 1.0 pg/mL
Range 3.9-250 pg/mL
Reacted Species Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Human plamsa93%80%-109%
Cell culture supernatants92%77%-107%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120206.112.15.9
22040.72.86.9
3205.30.47.3

InterAssay

Samplenmean ( pg/mL)SDCV%
124272.820.97.7
22472.34.15.7
32411.81.08.8

Background Information

CSF2, also named as GM-CSF, is an important hematopoietic growth factor and immune modulator, which is produced by a variety of cell types including T cells, macrophages, endothelial cells and fibroblasts upon receiving immune stimuli. It was originally recognized as a stimulator for the proliferation of granulocytes and macrophages from bone marrow precursor cells. It has also been shown to promote the survival and activation of mature myeloid cells and therefore contributes to the maintenance of innate immune homeostasis. Recent studies suggest that GM-CSF also has proinflammatory functions and plays critical roles in the development of autoimmune and inflammatory diseases, particularly in Th17 driven diseases. GM-CSF also plays a role in embryonic development by functioning as an embryokine produced by reproductive tract.

Properties

Storage Instructions All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms Colony stimulating factor, CSF, CSF2, GM CSF, GMCSF, Molgramostin, Sargramostim