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Recombinant Human Haptoglobin/HP protein (His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg0445

Validation Data Gallery

  • Purity of Recombinant Human Haptoglobin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human Haptoglobin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human Haptoglobin protein Val19-Asn406 (Accession# P00738-1) with a His tag at the C-terminus.
GeneID 3240
Accession P00738-1
PredictedSize 47.1 kDa
SDS-PAGE 17 kDa, 36-46 kDa and 55-65 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Haptoglobin (Hp) is an abundant human plasma protein that tightly captures hemoglobin (Hb) during hemolysis. The Hb-Hp complex formation reduces the oxidative properties of heme/Hb and promotes recognition by the macrophage scavenger receptor CD163. The major functions of Hp are to scavenge Hb, prevent iron loss, and prevent heme-based oxidation. Decreased haptoglobin is a marker for hemolysis because haptoglobin levels become depleted in the presence of large amounts of free hemoglobin.

References:

1.Andersen, Christian Brix Folsted et al. (2017) Antioxid Redox Signal.26(14):814-831. 
2.Shih, Andrew W Y et al. (2014) Am J Hematol.89(4):443-447. 
3.Delanghe, Joris R et al. (2024) Crit Rev Clin Lab Sci.61(4):275-297.