Human IL-22 ELISA Kit

Sensitivity

14.2 pg/mL

Range

31.25-2000 pg/mL

Reacted Species

Human

Cat no : KE00008

Synonyms

Cytokine Zcyto18, IL 21, IL 22, IL D110, IL TIF, IL21, IL22, IL-22, ILTIF, interleukin 22, TIFa, TIFIL 23, zcyto18



Product Information

KE00008 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL22 ELISA kit is to be used to detect and quantify protein levels of endogenous IL22. The assay recognizes human IL22. An antibody specific for IL22 has been pre-coated onto the microwells. The IL22 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL22 is added to detect the captured IL22 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Product name Human IL-22 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 14.2 pg/mL
Range 31.25-2000 pg/mL
Reacted Species Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Human plamsa113%108%-121%
Cell culture supernatants114%101%-119%

IntraAssay

Samplenmean ( pg/mL)SDCV%
1202035.370.93.5
220539.226.44.9
32054.83.66.5

InterAssay

Samplenmean ( pg/mL)SDCV%
1242174.7155.37.1
224577.546.88.1
32459.74.77.9

Background Information

Interleukin 22 (IL22) is a member of the human type I interferon family, which includes IL10. IL22 has the potential to interact with IL10 because it binds to the IL-10R2c chain with IL22R1 in its receptor complex. Binding can be blocked by the soluble receptor, IL22 binding protein (IL22BP). Although known as a Th17 cytokine, IL22 is also expressed by a wide range of immune cells, including NK T, γδ T, and NK cells. IL22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. IL22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL10.

Properties

Storage Instructions All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms Cytokine Zcyto18, IL 21, IL 22, IL D110, IL TIF, IL21, IL22, IL-22, ILTIF, interleukin 22, TIFa, TIFIL 23, zcyto18