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Recombinant Human IL4 protein (Myc Tag, His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

Myc Tag, His Tag

Activity

not tested

Cat no : Eg0104

Validation Data Gallery

  • Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.5-2ng/mL.

    Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.5-2ng/mL.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <1.0 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human IL4 protein His25-153Ser (Accession# P05112) with a Myc tag and a His tag at the C-terminus.
GeneID 3565
Accession P05112
PredictedSize 20.6 kDa
SDS-PAGE
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

IL-4 is a cytokine produced by CD4+ T cells in response to helminthes and other extracellular parasites. It promotes the proliferation and differentiation of antigen presenting cells. IL4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.

References:

1. Bankaitis, Katherine Venmar, et al. Clinical & experimental metastasis vol. 32,8 (2015): 847-56.
2. Hassan, Mir Tanveerul et al. Methods (San Diego, Calif.) vol. 217 (2023): 49-56. 
3. Tang, M L, and A S Kemp. Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology vol. 6,1 (1995): 11-9.