Human IL-6 ELISA Kit
Cat no : KE00007
Synonyms
IL6
Validation Data Gallery
Product Information
KE00007 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL6 ELISA kit is to be used to detect and quantify protein levels of endogenous IL6. The assay recognizes human IL6. An antibody specific for IL6 has been pre-coated onto the microwells. The IL6 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for IL6 is added to detect the captured IL6 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
| Product name | Human IL-6 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Urine |
| Assay type | Sandwich |
| Sensitivity | 3.8 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3569 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Urine | 99% | 80%-116% |
| Cell culture supernatants | 107% | 99%-114% |
| Human plasma | 92% | 78%-122% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 587.3 | 35.0 | 6.0 |
| 2 | 20 | 123.0 | 9.9 | 8.1 |
| 3 | 20 | 25.3 | 1.9 | 7.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 622.3 | 28.7 | 4.6 |
| 2 | 24 | 102.7 | 7.1 | 6.9 |
| 3 | 24 | 24.0 | 1.3 | 5.4 |
Background Information
Interleukin-6 (IL6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL6 Plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL6 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Sci Signal The suppressive effects of Mer inhibition on inflammatory responses in the pathogenesis of LPS-induced ALI/ARDS. | ||
J Nutr Biochem Can the dietary fat type facilitate memory impairments in adulthood? A comparative study between Mediterranean and Western-based diet in rats. | ||
Cell Prolif Long non-coding RNA 01126 promotes periodontitis pathogenesis of human periodontal ligament cells via miR-518a-5p/HIF-1α/MAPK pathway. | ||
Eur J Med Chem The discovery, design and synthesis of potent agonists of adenylyl cyclase type 2 by virtual screening combining biological evaluation. | ||
J Clin Periodontol Periodontitis and systemic markers of neurodegeneration: A case-control study. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Kasper (Verified Customer) (08-27-2019) | Human IL-6 Elisa kit was used to determine IL-6 levels in cell culture supernatants as per the instructions. The kit worked quite well and was very easy to use.
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