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Recombinant Human KIR2DL3 protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2699

Validation Data Gallery

  • Purity of Recombinant Human KIR2DL3 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human KIR2DL3 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human KIR2DL3 protein His22-His245 (Accession# P43628-1) with a rabbit IgG Fc tag at the C-terminus.
GeneID 3804
Accession P43628-1
PredictedSize 50.6 kDa
SDS-PAGE 60-75 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Killer cell immunoglobulin-like receptors (KIRs) are a diverse family of inhibitory and activating receptors expressed on NK cells and a subset of T cells. These polymorphic receptors interact with specific motifs on HLA class I molecules, modulate NK cytolytic activity, and are encoded by genes located on chromosome 19q13.4. KIR2DL3, also known as CD158B2 or NKAT2, is an inhibitory receptor that is specific for HLA-C alleles (HLA-Cw1, HLA-Cw3, and HLA-Cw7). It is a 341-amino acid transmembrane glycoprotein consisting of an extracellular region containing two C2-type Ig-like domains, a 19-amino acid hydrophobic transmembrane region, and a long cytoplasmic tail with two immunoreceptor tyrosine-based inhibitory motifs (ITIMs). KIR2DL3 inhibits the cytolytic activity of NK cells thus preventing cell lysis.

References:

1. Carlos Vilches. et al. (2002). Annu Rev Immunol. 20:217-251.
2. A Bontadini. et al. (2006). J Transl Med. 4:44.
3. K Maenaka. et al. (1999). Structure. 7(4):391-398.
4. M Vitale. et al. (1995). Proc Natl Acad Sci U S A. 92(8):3536-3540.