Human LEPR ELISA Kit
Cat no : KE00628
Synonyms
CD295, DB, HuB219, LEP R, LEP-R
Validation Data Gallery
Product Information
KE00628 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human LEPR ELISA kit is to be used to detect and quantify protein levels of endogenous human LEPR. The assay recognizes human LEPR. An antibody specific for human LEPR has been pre-coated onto the microwells. The human LEPR protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human LEPR is added to detect the captured human LEPR protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human LEPR ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.04 ng/mL |
| Range | 0.47-30 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3953 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 107% | 102%-111% |
| Cell culture supernatant | 96% | 80%-102% |
| Cell lysate | 94% | 71%-105% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 15.51 | 0.64 | 4.15 |
| 2 | 8 | 3.65 | 0.29 | 7.83 |
| 3 | 8 | 2.05 | 0.20 | 9.83 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 15.89 | 1.03 | 6.50 |
| 2 | 16 | 3.68 | 0.25 | 6.70 |
| 3 | 16 | 2.01 | 0.20 | 10.02 |
Background Information
The hormone leptin, secreted predominantly from adipose tissue, plays a crucial role in the regulation of numerous neuroendocrine functions, from energy homeostasis to reproduction . Leptin acts through the leptin receptor (LEPR, also known as OB-R), a single-transmembrane-domain receptor of the cytokine receptor family. On ligand binding, LEPR mediates signaling through JAK2/STAT3. LEPR plays an important role in the regulation of glucose homeostasis via feeding and adiposity.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD295, DB, HuB219, LEP R, LEP-R |