Recombinant Human LIF protein (Myc & 6*His)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
Myc Tag, His Tag
Activity
not tested
Cat no : Eg0111
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <1.0 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human LIF protein Ser23-Phe202 (Accession# P15018) with a Myc tag and a His tag at the C-terminus. |
| GeneID | 3976 |
| Accession | P15018 |
| PredictedSize | 25.3 kDa |
| SDS-PAGE | |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
LIF is a pleiotropic cytokine with roles in several different systems. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. LIF is a glycoprotein containing six putative N-glycosylation sites. The molecular weight of the unglycosylated LIF protein is 20-25 kDa, while the molecular weight of the glycosylated protein is in the range of 37-63 kDa.
References:
1. Viswanadhapalli, Suryavathi et al. Genes & diseases vol. 9,4 973-980. 29 Apr. 2. Nicola, Nicos A, and Jeffrey J Babon. Cytokine & growth factor reviews vol. 26,5 (2015): 533-44. 3. Hilton, D J. Trends in biochemical sciences vol. 17,2 (1992): 72-6. 4. Fan, L et al. Journal of dental research vol. 101,1 (2022): 63-72.