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Recombinant Human LY75/CD205 protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg3439

Validation Data Gallery

  • Purity of Recombinant Human LY75 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human LY75 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human LY75 protein Ser28-Asp1666 (Accession# O60449-1) with a rabbit IgG Fc tag at the C-terminus.
GeneID 4065
Accession O60449-1
PredictedSize 215.1 kDa
SDS-PAGE 170-250 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

LY75 (also known as CD205) is a type I transmembrane surface protein, which is mainly expressed on dendritic cells (DCs) and thymic cortical epithelial cells. LY75 is a member of the MMR family of multidomain molecules. It contains a cysteine rich N-terminal domain, a fibronectin type II domain, and multiple C-type lectin-like domains. LY75/CD205 serves as an endocytosis receptor involved in antigen uptake, presentation, and initiation of the immune response. LY75/CD205 plays an important role in antigen uptake and presentation and T cell activation.

References:

1. W Jiang, et al. (1995) Nature. 375(6527):151-5.
2. Matt Butler, et al. (2007) Immunology. 120(3):362-71.
3. Minchan Gil, et al. (2020) J Clin Med. 9(5):1383.