Human MDM2/HDM2 ELISA Kit
Cat no : KE00864
Synonyms
MDM2, E3 ubiquitin-protein ligase Mdm2, EC:2.3.2.27, hdm2, p53-binding protein Mdm2
Validation Data Gallery
Product Information
KE00864 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human MDM2/HDM2 ELISA kit is to be used to detect and quantify protein levels of endogenous human MDM2/HDM2. The assay recognizes human MDM2/HDM2. An antibody specific for human MDM2/HDM2 has been pre-coated onto the microwells. The human MDM2/HDM2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human MDM2/HDM2 is added to detect the captured human MDM2/HDM2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human MDM2/HDM2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.05 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4193 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysate | 91% | 87%-97% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 5.67 | 0.09 | 1.58 |
| 2 | 8 | 1.30 | 0.02 | 1.83 |
| 3 | 8 | 0.74 | 0.02 | 2.69 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 5.48 | 0.23 | 4.11 |
| 2 | 16 | 1.32 | 0.03 | 2.58 |
| 3 | 16 | 0.71 | 0.04 | 5.02 |
Background Information
MDM2, also known as HDM2, is a protein of the E3 ubiquitin-ligase family. MDM2 is recognized as a proto-oncogene and is over-expressed in a wide range of human malignancies including soft tissue carcinomas and breast cancer. MDM2 can bind to the p53 tumor suppressor protein with high affinity, and negatively regulates p53 by mediating its ubiquitination and proteosomal degradation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | MDM2, E3 ubiquitin-protein ligase Mdm2, EC:2.3.2.27, hdm2, p53-binding protein Mdm2 |