Recombinant Human MICA protein (Myc & 6*His)
Species
Human
Purity
>90 %, SDS-PAGE
Tag
Myc Tag, His Tag
Activity
not tested
Cat no : Eg0181
Validation Data Gallery
Product Information
| Purity | >90 %, SDS-PAGE |
| Endotoxin | <1.0 EU/μg protein, LAL method |
| Activity |
Not tested |
| Expression | HEK293-derived Human MICA protein Glu24-Gln308 (Accession# BC016929) with a Myc tag and a His tag at the C-terminus. |
| GeneID | 100507436 |
| Accession | BC016929 |
| PredictedSize | 38.4 kDa |
| SDS-PAGE | |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
| Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
| Storage Conditions |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides. They are expressed in intestinal epithelium and many epithelial tumors. MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells.
References:
1. Stephens, H A. Trends in immunology vol. 22,7 (2001): 378-85. 2. Groh, V et al. Science (New York, N.Y.) vol. 279,5357 (1998): 1737-40. 3. Groh, V et al. Proceedings of the National Academy of Sciences of the 4. United States of America vol. 96,12 (1999): 6879-84. 5. Bauer, S et al. Science (New York, N.Y.) vol. 285,5428 (1999): 727-9. 6. Steinle, A et al. Immunogenetics vol. 53,4 (2001): 279-87.