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Recombinant Human MICA protein (His Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

His Tag

Activity

not tested

Cat no : Eg1176

Validation Data Gallery

  • Purity of Recombinant Human MICA was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human MICA was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human MICA protein Glu24-Gln308 (Accession# AAH16929.1) with a His tag at the C-terminus.
GeneID 4276
Accession AAH16929.1
PredictedSize 33.6 kDa
SDS-PAGE 45-60 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides. They are expressed in intestinal epithelium and many epithelial tumors. MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells.

References:

1. V Groh, et al. (1998) Science. 279(5357):1737-40.
2. V Groh, et al. (1999) Proc Natl Acad Sci U S A. 96(12):6879-84.
3. S Bauer, et al. (1999) Science. 285(5428):727-9.
4. H A Stephens. (2001) Trends Immunol. 22(7):378-85.
5. A Steinle, et al. (2001) Immunogenetics. 53(4):279-87.