Human Total MMP-3 ELISA Kit
Cat no : KE00094
Synonyms
CHDS6, Matrix metalloproteinase 3, MMP 3, MMP3, MMP-3, SL 1, STMY, STMY1, STR1, Stromelysin 1, Transin 1
Validation Data Gallery
Product Information
KE00094 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The MMP-3 ELISA kit is to be used to detect and quantify protein levels of endogenous MMP-3. The assay recognizes human MMP-3 . A polyclonal antibody specific for MMP-3 has been pre-coated onto the microwells. The MMP-3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for MMP-3 is added to detect the captured MMP-3 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Total MMP-3 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Plasma, Serum, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.4 - 96 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4314 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Plasma | 85% | 76%-101% |
| Cell culture supernatant | 85% | 70%-111% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | ? ug/ml | ? | ?% |
| 2 | 20 | ? ug/ml | ? | ?% |
| 3 | 20 | ? ug/ml | ? | ?% |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | ? ug/ml | ? | ?% |
| 2 | 20 | ? ug/ml | ? | ?% |
| 3 | 20 | ? ug/ml | ? | ?% |
Background Information
Matrix metalloproteinases (MMPs) play a critically important role in extracellular matrix remodeling and have been implicated in a number of key normal and pathologic processes.These proteases have come to represent important therapeutic and diagnostic targets for the treatment and detection of human cancers. MMP-3 activate procollagenase via two pathways: slow direct activation and rapid activation in conjunction with tissue or plasma proteinases. The pro-MMP3 (60 kDa) and the active MMP3 (47 kDa) can be detected through western blot.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CHDS6, Matrix metalloproteinase 3, MMP 3, MMP3, MMP-3, SL 1, STMY, STMY1, STR1, Stromelysin 1, Transin 1 |