Human P21 ELISA Kit

Sensitivity

0.1 ng/mL

Range

0.25-16 ng/mL

Reactivity

Human

Cat no : KE00049

Synonyms

CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, P21;CDKN1A, p21CIP1, SDI1, WAF1



Product Information

KE00049 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The P21 ELISA kit is designed for the quantitative determination of P21 in samples of human and mouse origin. An antibody specific for P21 has been pre-coated onto the microwells. The P21 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for P21 is added to detect the captured P21 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Product name Human P21 ELISA Kit
Tests 1 X 96 well plate
Sample type Cell lysates
Assay type Sandwich
Sensitivity 0.1 ng/mL
Range 0.25-16 ng/mL
Reactivity Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Cell lysates87%81%-97%

IntraAssay

Samplenmean ( ng/mL)SDCV%
12010.290.2012.0
2203.050.0702.3
3200.630.0254.0

InterAssay

Samplenmean ( ng/mL)SDCV%
12410.240.3463.4
2243.080.0943.1
3240.670.0527.8

Background Information

CDKN1A (p21, CIP1, WAF1), a cyclin-dependent kinase inhibitor, is necessary for proper control of the cell cycle and premature senescence. p21 is a cyclin-dependent kinase (CDK) inhibitor that suppresses proliferation by inhibiting CDK2 and CDK1 activity at the G1/S and G2/M transitions. p21 is a major mediator of p53 to induce cell cycle arrest in G1. p21 can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. p21 was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Overexpression of p21 decreases the expression levels of cell cycle progression genes and upregulates senescence-inducing genes.

Properties

Storage Instructions All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, P21;CDKN1A, p21CIP1, SDI1, WAF1

Publications

SpeciesSample TypeTitle
humanCell lysate

Analyst

Sensitive and selective monitoring of the DNA damage-induced intracellular p21 protein and unraveling the role of the p21 protein in DNA repair and cell apoptosis by surface plasmon resonance.

Authors - Yuhan He