Human P21 ELISA Kit

Sensitivity

0.1 ng/mL

Range

0.25-16 ng/mL

Reactivity

Human

Cat no : KE00049

Synonyms

CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, P21;CDKN1A, p21CIP1, SDI1, WAF1



Product Information

KE00049 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The P21 ELISA kit is designed for the quantitative determination of P21 in samples of human origin. An antibody specific for P21 has been pre-coated onto the microwells. The P21 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for P21 is added to detect the captured P21 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human P21 ELISA Kit
Tests 1 X 96 well plate
Sample type Cell lysates
Assay type Sandwich
Sensitivity 0.1 ng/mL
Range 0.25-16 ng/mL
Reactivity Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Cell lysates87%81%-97%

IntraAssay

Samplenmean ( ng/mL)SDCV%
12010.290.2012.0
2203.050.0702.3
3200.630.0254.0

InterAssay

Samplenmean ( ng/mL)SDCV%
12410.240.3463.4
2243.080.0943.1
3240.670.0527.8

Background Information

P21 (also known as CIP1, WAF1) is a cyclin-dependent kinase inhibitor. P21 binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of P21 is induced by wild-type but not mutant p53 protein, through which P21 mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. P21 can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. P21 was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms CAP20, CDK interacting protein 1, CDKN1, CDKN1A, CIP1, MDA 6, MDA6, P21, P21;CDKN1A, p21CIP1, SDI1, WAF1

Publications

SpeciesSample TypeTitle
humanCell lysate

Analyst

Sensitive and selective monitoring of the DNA damage-induced intracellular p21 protein and unraveling the role of the p21 protein in DNA repair and cell apoptosis by surface plasmon resonance.

Authors - Yuhan He
humanserum

Biomolecules

Correlation of Occupational Exposure to Carcinogenic Polycyclic Aromatic Hydrocarbons (cPAHs) and Blood Levels of p53 and p21 Proteins.

Authors - Saleh A K Saleh