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Human PD-L1 ELISA Kit1 Publications

Catalog number: KE00074

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$399.00

Freight/Packing £00.00

Size: 09µ/gram

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Overview

Product name:
Human PD-L1 ELISA Kit
Tests:
1 X 96 well plate
Sample type:
Serum, Plasma, Cell culture supernatants, Cell lysates
Assay type:
Sandwich
Sensitivity:
0.04 ng/mL
Range:
0.156-10 ng/mL
Reacted Species:
Human
Tested applications:
Sandwich ELISA
Recovery:
Sample TypeAverageRange
Human plasma 94% 74%-125%
Cell culture supernatants 105% 87%-127%
Cell lysates 100% 74%-126%
IntraAssay:
Samplenmean ( ng/mL)SDCV%
1 20 8.26 0.64 7.8
2 20 2.02 0.14 6.8
3 20 0.49 0.02 4.6
InterAssay:
Samplenmean ( ng/mL)SDCV%
1 24 7.43 0.38 5.1
2 24 1.90 0.12 6.1
3 24 0.47 0.03 7.0
Product overview:
KE00074 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The PD-L1 ELISA kit is to be used to detect and quantify protein levels of endogenous PD-L1. The assay recognizes human PD-L1. A polyclonal antibody specific for PD-L1 has been pre-coated onto the microwells. The PD-L1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for PD-L1 is added to detect the captured PD-L1 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Properties

Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms:
B7 H, B7 H1, B7 homolog 1, B7H1, CD274, CD274 molecule, PD L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL1, PD-L1/CD274, Programmed death ligand 1
Background

PD-L1 (programmed cell death ligand 1, also known as CD274 or B7-H1) is a 290 aa type I transmembrane protein. PD-L1 is expressed constitutively on murine T cells, B cells, DCs, macrophages, mesenchymal stem cells and cultured bone marrow-derived mast cells. In addition, PD-L1 is also expressed on many nonhematopoietic cell types, including vascular endothelial cells, epithelial cells, muscle cells, hepatocytes, pancreatic islet cells, astrocytes in the brain, placental syncytiotrophoblasts, and cells in cornea, iris-ciliary body and retina of eye. PD-L1 is frequently upregulated in a wide variety of solid tumors, including melanoma, ovarian, lung, glioblastoma, breast, and pancreatic cancers. PD-L1 and PD-L2 are two ligands of PD-1. Engagement of PD-1 by PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function. It is critical for the regulation of T cell function during tolerance, autoimmunity and infection.


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