Human PD-L1 ELISA Kit
Cat no : KE00074
Synonyms
B7 H, B7 H1, B7 homolog 1, B7H1, CD274, CD274 molecule, PD L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL1, PD-L1/CD274, Programmed death ligand 1
Validation Data Gallery
Product Information
KE00074 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The PD-L1 ELISA kit is to be used to detect and quantify protein levels of endogenous PD-L1. The assay recognizes human PD-L1. An antibody specific for PD-L1 has been pre-coated onto the microwells. The PD-L1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for PD-L1 is added to detect the captured PD-L1 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
| Product name | Human PD-L1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Cell lysates |
| Assay type | Sandwich |
| Sensitivity | 0.04 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 94% | 74%-125% |
| Cell culture supernatants | 105% | 87%-127% |
| Cell lysates | 100% | 74%-126% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 8.26 | 0.64 | 7.8 |
| 2 | 20 | 2.02 | 0.14 | 6.8 |
| 3 | 20 | 0.49 | 0.02 | 4.6 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 7.43 | 0.38 | 5.1 |
| 2 | 24 | 1.90 | 0.12 | 6.1 |
| 3 | 24 | 0.47 | 0.03 | 7.0 |
Background Information
PD-L1 (programmed cell death ligand 1, also known as CD274 or B7-H1) is a 290 aa type I transmembrane protein. PD-L1 is expressed constitutively on murine T cells, B cells, DCs, macrophages, mesenchymal stem cells and cultured bone marrow-derived mast cells. In addition, PD-L1 is also expressed on many nonhematopoietic cell types, including vascular endothelial cells, epithelial cells, muscle cells, hepatocytes, pancreatic islet cells, astrocytes in the brain, placental syncytiotrophoblasts, and cells in cornea, iris-ciliary body and retina of eye. PD-L1 is frequently upregulated in a wide variety of solid tumors, including melanoma, ovarian, lung, glioblastoma, breast, and pancreatic cancers. PD-L1 and PD-L2 are two ligands of PD-1. Engagement of PD-1 by PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function. It is critical for the regulation of T cell function during tolerance, autoimmunity and infection.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions. |
| Synonyms | B7 H, B7 H1, B7 homolog 1, B7H1, CD274, CD274 molecule, PD L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL1, PD-L1/CD274, Programmed death ligand 1 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Crit Care Lymphocyte subset expression and serum concentrations of PD-1/PD-L1 in sepsis - pilot study. |