Human PD-L2/B7-DC ELISA Kit

Sensitivity

3.8 pg/mL

Range

15.6-1000 pg/mL

Reactivity

Human

Cat no : KE00347

Synonyms

B7 DC, B7DC, bA574F11.2, Btdc, Butyrophilin B7 DC, CD273, PD 1 ligand 2, PD L2, PDCD1 ligand 2, PDCD1L2, PDCD1LG2, PDL2, Programmed death ligand 2



Product Information

KE00347 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human PD-L2/B7-DC ELISA kit is to be used to detect and quantify protein levels of endogenous human PD-L2/B7-DC. The assay recognizes human PD-L2/B7-DC. An antibody specific for human PD-L2/B7-DC has been pre-coated onto the microwells. The human PD-L2/B7-DC protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human PD-L2/B7-DC is added to detect the captured human PD-L2/B7-DC protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human PD-L2/B7-DC ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatant, Urine, Human milk
Assay type Sandwich
Sensitivity 3.8 pg/mL
Range 15.6-1000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 80380

Recovery

Sample TypeAverageRange
Human serum95%75%-114%
Urine100%89%-115%
Cell culture supernatant94%77%-127%
Human milk101%86%-128%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120969.146.24.8
220245.214.15.8
32046.24.59.7

InterAssay

Samplenmean ( pg/mL)SDCV%
1241,116.664.35.8
224247.916.26.5
32456.95.69.8

Background Information

Programmed cell death ligand 2 (PD-L2, also known as CD273, or B7-DC), is one of the B7 family molecules which are type I transmembrane proteins belonging to the immunoglobulin superfamily. PD-L2 was identified both by its homology to PD-L1 and by analysis of genes differentially expressed in libraries generated from dendritic cells (DC) and activated macrophages. PD-L2 is a second ligand for PD-1, a costimulatory molecule that plays an inhibitory role in regulating T-cell activation. Overexpression of PD-L2 has been reported in malignant tumors and significantly contributes to tumorigenesis and cancer progression.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms B7 DC, B7DC, bA574F11.2, Btdc, Butyrophilin B7 DC, CD273, PD 1 ligand 2, PD L2, PDCD1 ligand 2, PDCD1L2, PDCD1LG2, PDL2, Programmed death ligand 2