Human PD1 ELISA Kit

Sensitivity

43.0 pg/mL

Range

125-8000 pg/mL

Reactivity

Human

Cat no : KE00075

Synonyms

CD279, hPD 1, hPD l, PD1, PD-1/CD279, PDCD1, programmed cell death 1, Protein PD 1, SLEB2



Product Information

KE00075 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The PD1 ELISA kit is to be used to detect and quantify protein levels of endogenous PD1. The assay recognizes human PD1. An antibody specific for PD1 has been pre-coated onto the microwells. The PD1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for PD1 is added to detect the captured PD1 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Product name Human PD1 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants, Cell lysates
Assay type Sandwich
Sensitivity 43.0 pg/mL
Range 125-8000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Human plasma96%80%-116%
Cell lysates101%83%-120%
Cell culture supernatants95%79%-120%

IntraAssay

Samplenmean ( pg/mL)SDCV%
1207,3901912.6
2202,176813.7
320542142.6

InterAssay

Samplenmean ( pg/mL)SDCV%
1247,7963824.9
2242,3991395.8
324539275.0

Background Information

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM). PD-1 can be expressed on T cells, B cells, natural killer T cells, activated monocytes, and dendritic cells (DCs). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function. It is critical for the regulation of T cell function during tolerance, autoimmunity and infection. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity.

Properties

Storage Instructions All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms CD279, hPD 1, hPD l, PD1, PD-1/CD279, PDCD1, programmed cell death 1, Protein PD 1, SLEB2

Publications

SpeciesSample TypeTitle
humanserum

Crit Care

Lymphocyte subset expression and serum concentrations of PD-1/PD-L1 in sepsis - pilot study.

Authors - Julie K Wilson