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Human Pre-IL18 Kit0 Publications

Catalog number: KE00025

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$399.00

Freight/Packing £00.00

Size: 09µ/gram

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Overview

Product name:
Human Pre-IL18 Kit
Tests:
1 X 96 well plate
Sample type:
Serum, Plasma
Assay type:
Sandwich
Sensitivity:
1.5 pg/mL
Range:
15.6-1000 pg/mL
Reacted Species:
Human
Tested applications:
Sandwich ELISA
Recovery:
Sample TypeAverageRange
Human plasma 87% 77%-110%
IntraAssay:
Samplenmean ( pg/mL)SDCV%
1 20 512.7 18.4 3.6
2 20 265.8 9.9 3.7
3 20 89.9 5.5 6.1
InterAssay:
Samplenmean ( pg/mL)SDCV%
1 24 495.5 20.6 4.2
2 24 258.4 13.3 5.2
3 24 90.9 4.2 4.7
Product overview:
Human IL-18 Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-18 ELISA kit is to be used to detect and quantify protein levels of endogenous IL-18. The assay recognizes human IL-18. A polyclonal antibody specific for IL-18 has been pre-coated onto the microwells. The IL-18 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for IL-18 is added to detect the captured IL-18 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties

Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.
Synonyms:
Iboctadekin, IFN gamma inducing factor, IGIF, IL 1 gamma, IL 18, IL 1g, IL18, IL-18, IL1F4, Interleukin 1 gamma, Interleukin 18, Pre-IL18
Background

IL18, also named as IGIF and IL1F4, belongs to the IL-1 family. It augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. The IL18 cytokine increased expression of vascular cell adhesion molecule-1(VCAM1) and the adherence of melanoma cells. IL18 plays a major role in atherosclerotic plaque destabilization leading to acute ischemic syndromes. IL18 negatively regulate NAFLD/NASH progression, as well as multiple aspects of metabolic syndrome via modulation of the gut microbiota. This kit is used to quantify IL18 level in vivo.


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