Human Total IL-18 ELISA Kit
Cat no : KE00025
Synonyms
Iboctadekin, IGIF, IL 1 gamma, IL 18, IL 1g, IL18, IL-18, IL1F4, Interleukin 1 gamma, Interleukin 18, Pre-IL18
Validation Data Gallery
Product Information
KE00025 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL18 ELISA kit is to be used to detect and quantify protein levels of endogenous Pre-IL18 and mature IL18. The assay recognizes human IL18. An antibody specific for IL18 has been pre-coated onto the microwells. The IL18 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for IL18 is added to detect the captured IL18 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.
| Product name | Human Total IL-18 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 1.5 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3606 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 87% | 77%-110% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 512.7 | 18.4 | 3.6 |
| 2 | 20 | 265.8 | 9.9 | 3.7 |
| 3 | 20 | 89.9 | 5.5 | 6.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 495.5 | 20.6 | 4.2 |
| 2 | 24 | 258.4 | 13.3 | 5.2 |
| 3 | 24 | 90.9 | 4.2 | 4.7 |
Background Information
IL-18 is a member of IL-1 superfamily of cytokines. IL-18 is expressed by many human lymphoid and nonlymphoid cells, and has an important role in inflammatory processes. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL-18 synergistically with IL-12 contributes to Th1 differentiation and, therefore, is important in host defense mechanisms against intracellular bacteria, viruses, and fungi. The IL-18 cytokine increased expression of vascular cell adhesion molecule-1(VCAM1) and the adherence of melanoma cells. IL-18 plays a major role in atherosclerotic plaque destabilization leading to acute ischemic syndromes. IL-18 negatively regulate NAFLD/NASH progression, as well as multiple aspects of metabolic syndrome via modulation of the gut microbiota. This kit is used to quantify Pre-IL18 and mature IL18 level in vivo.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Iboctadekin, IGIF, IL 1 gamma, IL 18, IL 1g, IL18, IL-18, IL1F4, Interleukin 1 gamma, Interleukin 18, Pre-IL18 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Cell Death Dis N6-methyladenosine modification of circCUX1 confers radioresistance of hypopharyngeal squamous cell carcinoma through caspase1 pathway. | ||
Biomedicines Ruscogenin Attenuates Ulcerative Colitis in Mice by Inhibiting Caspase-1-Dependent Pyroptosis via the TLR4/NF-κB Signaling Pathway | ||
J Neuroinflammation Glutamine metabolism modulates microglial NLRP3 inflammasome activity through mitophagy in Alzheimer's disease | ||
Heliyon Conditional reprogrammed human limbal epithelial cell model for anti-SARS-CoV-2 drug screening |