Recombinant Human Neuroserpin protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2819



Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity
Not tested
Expression HEK293-derived Human Neuroserpin protein Thr17-Leu410 (Accession# Q99574) with a rabbit IgG Fc tag at the C-terminus.
GeneID 5274
Accession Q99574
PredictedSize 70.7 kDa
SDS-PAGE 75-90 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Neuroserpin is a member of the serpin family of serine protease inhibitors predominantly expressed in the nervous system, such as s the cerebral cortex, hippocampus and the spinal cord.Neuroserpin exerts protective effects in neurons against excitotoxicity both in vitro and in vivo. Its proteolytic inhibitory activity is shown to protect neurons against damage caused by plasmin activation. Mice overexpressing neuroserpin exhibited loss of tPA activity in the brain. Overexpression of neuroserpin in primary neurons leads to increased dendritic arborization and altered dendritic spine shape.

References:

1. Yang X, Fang Z, et al. (2022) Seizure. 103:137-147. 2. Matsuda Y, Miura K, et al. (2016) Cancer Sci. 107(5):619-28. 3. Mirshahvaladi S, Chitranshi N, et al. (2024) Proteomes. 12(1):7. 4. Ranza E, Garcia-Tarodo S, et al. (2017) Am J Med Genet A. 173(9):2456-2460.