Human SMN2 ELISA Kit

Catalog number: KE00027

$599.00

Freight/Packing £00.00

Size: 09µ/gram

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Overview


Product name:
Human SMN2 ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Tissue Lysate, Cell Lysate

Assay type:
Sandwich

Sensitivity:
6 pg/mL

Range:
62.5-4000 pg/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Cell lysate 113% 106-121%
Tissue lysate 90% 90-91%

Product overview:
KE00027 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The SMN2 ELISA kit is designed for the quantitative determination of SMN2 in samples of human and mouse origin. A polyclonal antibody specific for SMN2 has been pre-coated onto the microwells. The SMN2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for SMN2 is added to detect the captured SMN2 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
C BCD541, Component of gems 1, FLJ76644, Gemin 1, SMN, SMN1, SMN1,SMN, SMN2, SMNC, SMNT, Survival motor neuron protein
Background

Survival Motor Neuron proteins (SMN1 and SMN2) required for efficient assembly of small nuclear ribonucleoprotein (snRNP) complexes are encoded by nearly identical telomeric and centromeric forms of SMN gene respectively. Both the SMN1 and SMN2 genes express SMN protein, however, the amount of functional full-length protein produced by SMN2 is much less than that produced by SMN1 due to the alternative splicing.The SMN gene is constitutively expressed in wide variety of tissues including brain, kidney, liver and spinal cord, while motor neurons are particularly vulnerable to reduced SMN protein levels. Deletion or mutational inactivation of the SMN1 gene causes spinal muscular atrophy (SMA), a lethal genetic disease characterized by loss of motor neurons in the spinal cord. The absence of SMN1 can be partially compensated for by SMN2 and the SMN2 expression level is associated with SMA severity. Accurately measurement of SMN2 protein level may be used as an early 'biomarker' for assessing SMA treatment effect. This kit is for the quantitative determination of SMN2 level in vivo.


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