Human/Mouse/Rat SNAP25 ELISA Kit


6.0 pg/mL


15.6-1000 pg/mL



Cat no : KE00031


RIC 4, RIC4, SEC9, SNAP, SNAP 25, SNAP25, SUP, Super protein

Product Information

KE00031 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The SNAP25 ELISA kit is to be used to detect and quantify protein levels of endogenous SNAP25. The assay recognizes human/mouse/rat SNAP25. An antibody specific for SNAP25 has been pre-coated onto the microwells. The SNAP25 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for SNAP25 is added to detect the captured SNAP25 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human/Mouse/Rat SNAP25 ELISA Kit
Tests 1 X 96 well plate
Sample type Tissue lysate
Assay type Sandwich
Sensitivity 6.0 pg/mL
Range 15.6-1000 pg/mL
Reactivity Human/Mouse/Rat
Tested applications Sandwich ELISA
Gene ID (NCBI) 6616


Sample TypeAverageRange
Tissue lysate97%81%-119%


Samplenmean ( pg/mL)SDCV%


Samplenmean ( pg/mL)SDCV%

Background Information

Synaptic vesicle membrane docking and fusion is mediated by soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNARE) complex which is located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). Other than VAMP2 and STX1A, SNAP25 is another key component of SNARE core complex, and is known to be involved in regulating neurotransmitter release. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues and mutation of the cysteines abolishes palmitoylation and its membrane binding activity. As an important presynaptic plasma membrane protein, SNAP25 has been linked to memory and learning by its effect on long term potentiation in the hippocampus, thus playing a critical role in the synaptic function of specific neuronal systems. It has been reported that SNAP25 levels were were significantly decreased in brain tissue homogenates of patients with later stages of Alzheimer's disease compared with the controls, while the levels of SNAP25 were significantly increased in cerebrospinal fluid (CSF) of the group with an Alzheimer's disease biomarker profile than in the group with a control biomarker profile.


Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms RIC 4, RIC4, SEC9, SNAP, SNAP 25, SNAP25, SUP, Super protein


SpeciesSample TypeTitle
humancell culture supernatants

Alzheimers Dement

Blood neuro-exosomal synaptic proteins predict Alzheimer's disease at the asymptomatic stage.

Authors - Longfei Jia
humancell culture supernatants

Eur J Neurosci

Associations between cerebrospinal fluid markers and cognition in aging and dementia: A systematic review.

Authors - Tyler S Saunders


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Wilhelm (Verified Customer) (12-09-2020)

Unfortunataly there was a labelling on the frame of the strip holder as well as on 4 of the 12 strips and there was just one cover seal for the plates provided instead of three. But we already got the affirmation that we'll receive a new free kit for that reason. Additionally the kit didn't recognized the protein contained in our target samples...

  • Applications: Other
  • Cell Tissue Type: Mouse brain and serum, human serum and CSF