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Recombinant Human SPINT1 protein (rFc Tag) (HPLC verified)

Species

Human

Purity

>90 %, SDS-PAGE
>90 %, SEC-HPLC

Tag

rFc Tag

Activity

not tested

Cat no : Eg3131

Validation Data Gallery

  • Purity of Recombinant Human SPINT1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human SPINT1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • The purity of Human SPINT1 was greater than 90% as determined by SEC-HPLC.

    The purity of Human SPINT1 was greater than 90% as determined by SEC-HPLC.

Product Information

Purity >90 %, SDS-PAGE
>90 %, SEC-HPLC
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human SPINT1 protein Ala35-Glu465 (Accession# O43278-1) with a rabbit IgG Fc tag at the C-terminus.
GeneID 6692
Accession O43278-1
PredictedSize 73.8 kDa
SDS-PAGE 70-80 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

SPINT1, or Serine Peptidase Inhibitor, Kunitz Type 1, is a protein encoded by the SPINT1 gene in humans. SPINT1 functions as a potent inhibitor specific for HGF activator and is thought to be involved in the regulation of the proteolytic activation of HGF in injured tissues. It has been shown to modulate HGF/MET activation in certain cancer types, suggesting its role in tumor biology (PMID: 29532159). Furthermore, SPINT1 has been implicated in glucose homeostasis and insulin production. Studies have shown that SPINT1 disruption in mouse pancreas leads to glucose intolerance and impaired insulin production, involving the HEPSIN/MAFA signaling pathway (PMID: 39627229).

References:

1. Liu CL. et al. (2018). Histochem Cell Biol. 149(6):635-644.
2. Lin HH. et al. (2024). Nat Commun. 15(1):10537.