Human VEGF ELISA Kit

Sensitivity

6.5 pg/mL

Range

15.6-1000 pg/mL

Reactivity

Human

Cat no : KE00085

Synonyms

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF



Product Information

KE00085 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The VEGF ELISA kit is to be used to detect and quantify protein levels of endogenous VEGF. The assay recognizes human VEGF. An antibody specific for VEGF has been pre-coated onto the microwells. The VEGF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for VEGF is added to detect the captured VEGF protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

Product name Human VEGF ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 6.5 pg/mL
Range 15.6-1000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA

Recovery

Sample TypeAverageRange
Human plasma97%81%-115%
Cell culture supernatants108%96%-118%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120874.855.26.3
220246.114.25.8
32021.91.25.3

InterAssay

Samplenmean ( pg/mL)SDCV%
124963.969.17.2
224236.714.36.0
32424.61.97.6

Background Information

Vascular endothelial growth factor (VEGF), is a signal protein produced by cells that stimulates vasculogenesis and angiogenesis. It is part of the system that restores the oxygen supply to tissues when blood circulation is inadequate such as in hypoxic conditions. Serum concentration of VEGF is high in bronchial asthma and diabetes mellitus. The activities of VEGF are not limited to the vascular system; VEGF plays a role in normal physiological functions such as bone formation, hematopoiesis, wound healing, and development. VEGF is upregulated in many known tumors and its expression is correlated with tumor stage and progression.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF

Publications

SpeciesSample TypeTitle
humancell culture supernatants

Cancers (Basel)

A Novel Combination Cancer Therapy with Iron Chelator Targeting Cancer Stem Cells via Suppressing Stemness.

Authors - Yuki Katsura
humancell culture supernatants

J Exp Clin Cancer Res

Hypoxia-autophagy axis induces VEGFA by peritoneal mesothelial cells to promote gastric cancer peritoneal metastasis through an integrin α5-fibronectin pathway.

Authors - Xiaoxun Wang
humancell culture supernatants

Mol Cell Biochem

Anti-neovascularization effects of DMBT in age-related macular degeneration by inhibition of VEGF secretion through ROS-dependent signaling pathway.

Authors - Shang Chen
human

J Adv Res

33-kDa ANXA3 isoform contributes to hepatocarcinogenesis via modulating ERK, PI3K/Akt-HIF and intrinsic apoptosis pathways.

Authors - Chunmei Guo
humanserum

Evid Based Complement Alternat Med

Shenmayizhi Formula Combined with Ginkgo Extract Tablets for the Treatment of Vascular Dementia: A Randomized, Double-Blind, Controlled Trial.

Authors - Huiqin Zhang
humancell culture supernatants

Am J Transl Res

Fatty acid extracts facilitate cutaneous wound healing through activating AKT, ERK, and TGF-β/Smad3 signaling and promoting angiogenesis.

Authors - Junwei Zong