Human VEGF ELISA Kit
Cat no : KE00085
Synonyms
L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF
Validation Data Gallery
Product Information
KE00085 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The VEGF ELISA kit is to be used to detect and quantify protein levels of endogenous VEGF. The assay recognizes human VEGF. An antibody specific for VEGF has been pre-coated onto the microwells. The VEGF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for VEGF is added to detect the captured VEGF protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human VEGF ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 6.5 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7422 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 97% | 81%-115% |
| Cell culture supernatants | 108% | 96%-118% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 874.8 | 55.2 | 6.3 |
| 2 | 20 | 246.1 | 14.2 | 5.8 |
| 3 | 20 | 21.9 | 1.2 | 5.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 963.9 | 69.1 | 7.2 |
| 2 | 24 | 236.7 | 14.3 | 6.0 |
| 3 | 24 | 24.6 | 1.9 | 7.6 |
Background Information
Vascular endothelial growth factor (VEGF), is a signal protein produced by cells that stimulates vasculogenesis and angiogenesis. It is part of the system that restores the oxygen supply to tissues when blood circulation is inadequate such as in hypoxic conditions. Serum concentration of VEGF is high in bronchial asthma and diabetes mellitus. The activities of VEGF are not limited to the vascular system; VEGF plays a role in normal physiological functions such as bone formation, hematopoiesis, wound healing, and development. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. VEGF is upregulated in many known tumors and its expression is correlated with tumor stage and progression.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
Publications
| Species | Sample Type | Title |
|---|---|---|
Am J Transplant Enhanced autophagy alleviated corneal allograft rejection via inhibiting NLRP3 inflammasome activity. | ||
Front Cell Dev Biol Molecular and Clinicopathological Characterization of a Prognostic Immune Gene Signature Associated With MGMT Methylation in Glioblastoma. | ||
Mater Sci Eng C Mater Biol Appl Rubidium-containing mesoporous bioactive glass scaffolds support angiogenesis, osteogenesis and antibacterial activity. | ||
Drug Deliv Co-delivery of paclitaxel and anti-VEGF siRNA by tripeptide lipid nanoparticle to enhance the anti-tumor activity for lung cancer therapy. | ||
Int J Biol Sci Exosomal LBH inhibits epithelial-mesenchymal transition and angiogenesis in nasopharyngeal carcinoma via downregulating VEGFA signaling. | ||
Front Oncol Transmembrane Protein 100 Inhibits the Progression of Colorectal Cancer by Promoting the Ubiquitin/Proteasome Degradation of HIF-1α. |