Human VEGFR-1 ELISA Kit
Cat no : KE00113
Synonyms
FLT1,EC:2.7.10.1,FLT,FLT 1,FLT-1
Validation Data Gallery
Product Information
KE00113 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The VEGFR-1 ELISA kit is to be used to detect and quantify protein levels of endogenous VEGFR-1. The VEGFR-1 ELISA kit is designed to measure human soluble VEGFR-1 in serum, plasma and cell culture supernatant. The assay recognizes human VEGFR-1. An antibody specific for VEGFR-1 has been pre-coated onto the microwells. The VEGFR-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for VEGFR-1 is added to detect the captured VEGFR-1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human VEGFR-1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 7.8 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2321 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatants | 101% | 87%-113% |
| Human plasma | 98% | 80%-116% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 541.8 | 21.2 | 3.9 |
| 2 | 20 | 1,260.9 | 75.0 | 5.9 |
| 3 | 20 | 2,346.7 | 117.9 | 5.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 558.4 | 22.8 | 4.1 |
| 2 | 24 | 1,147.1 | 56.9 | 5.0 |
| 3 | 24 | 2,271.2 | 59.9 | 2.6 |
Background Information
Vascular endothelial growth factor receptor-1 (VEGFR-1, FLT-1) is a receptor tyrosine kinase belonging to the VEGFR family. VEGF is a key regulator of physiological angiogenesis and has also been implicated in pathological angiogenesis associated with tumors, intraocular neovascular disorders and other conditions. The biological effects of VEGF are mediated by VEGFR-1 and VEGFR-2. Both the two receptors have seven immunoglobulin-like repeats in the extracellular domain, a single transmembrane region and a tyrosine kinase domain. VEGFR-1 binds VEGFA, PIGF and VEGFB, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. Soluble VEGF receptor-1 (sVEGFR-1, sFlt-1), a truncated version of the cell membrane-spanning VEGFR-1, has the potential to act as a decoy receptor for VEGFA. Elevated serum sVEGFR-1 have been found in women with preeclampsia. Excess placental sVEGFR-1 may contribute to endothelial dysfunction, hypertension, and proteinuria in preeclampsia.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | FLT1,EC:2.7.10.1,FLT,FLT 1,FLT-1 |