Human VWF ELISA Kit
Cat no : KE00455
Synonyms
VWF,VWF, VWFpp,F8VWF,von Willebrand antigen II,VWD
Validation Data Gallery
Product Information
KE00455 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human VWF ELISA kit is to be used to detect and quantify protein levels of endogenous human VWF. The assay recognizes human VWF. An antibody specific for human VWF has been pre-coated onto the microwells. The human VWF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human VWF is added to detect the captured human VWF protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human VWF ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7450 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 91% | 87%-98% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 4.69 | 0.10 | 2.09 |
| 2 | 8 | 1.18 | 0.04 | 3.07 |
| 3 | 8 | 0.60 | 0.01 | 1.96 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 4.72 | 0.10 | 2.10 |
| 2 | 16 | 1.18 | 0.03 | 2.63 |
| 3 | 16 | 0.60 | 0.01 | 1.94 |
Background Information
Von Willebrand factor (VWF), the largest circulatory glycoprotein, supports shear stress-associated platelet adhesion and aggregation at sites of vascular injury. It is an array of multimeric (concatameric), disulfide‐linked, mirror‐symmetric glycoprotein dimers called protomers. The mature VWF monomer is a 2050‐residue protein (MW ~ 250 kDa) containing 12 N‐linked and 10 O‐linked oligosaccharide chains.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | VWF,VWF, VWFpp,F8VWF,von Willebrand antigen II,VWD |