IARS Polyclonal antibody

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  WB analysis using 26942-1-AP

  K-562 cells and U2OS cells were subjected to SDS PAGE followed by western blot with 26942-1-AP (IARS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 26942-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 26942-1-AP (IARS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 26942-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 26942-1-AP (IARS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of U2OS using 26942-1-AP

  IP result of anti-IARS (IP:26942-1-AP, 4ug; Detection:26942-1-AP 1:300) with U2OS cells lysate 1800 ug.

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  IHC staining of human testis using 26942-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26942-1-AP (IARS Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 26942-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26942-1-AP (IARS Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 26942-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26942-1-AP (IARS Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 26942-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26942-1-AP (IARS Antibody) at dilution of 1:200 (under 40x lens).

• Featured Product
• KD/KO Validated

IARS Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 26942-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

IARS, IARS1, IleRS, ILRS, IRS, Isoleucine tRNA ligase, isoleucyl tRNA synthetase, PRO0785

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Tested Applications

• Positive WB detected in: K-562 cells, U2OS cells
• Positive IP detected in: U2OS cells
• Positive IHC detected in: human testis tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 2 publications below

Product Information

26942-1-AP targets IARS in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IARS fusion protein Ag25686
• Full Name: isoleucyl-tRNA synthetase
• Calculated Molecular Weight: 144 kDa
• Observed Molecular Weight: 145 kDa
• GenBank Accession Number: BC065552
• Gene Symbol: IARS
• Gene ID (NCBI): 3376
• RRID: AB_2880693
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P41252
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Cell; A methyltransferase-independent role for METTL1 in tRNA aminoacylation and oncogenic transformation; Authors - Raja H Ali
rat	WB	Food Chem Toxicol; The crucial role of thioredoxin interacting protein in the liver insulin resistance induced by di (2-ethylhexyl) phthalates.; Authors - Wang Zhang
mouse	WB	Sci Rep; Intermittent fasting activates macrophage migration inhibitory factor and alleviates high-fat diet-induced nonalcoholic fatty liver disease; Authors - Dezhao Li
human	WB,IF	Discov Oncol; High dose isoleucine stabilizes nuclear PTEN to suppress the proliferation of lung cancer; Authors - Haiqing Wang
human	IF	Nat Commun; Time-resolved proteomic profiling reveals compositional and functional transitions across the stress granule life cycle; Authors - Shuyao Hu
human	WB	Proc Natl Acad Sci U S A; Multimodal cotranslational interactions direct assembly of the human multi-tRNA synthetase complex; Authors - Krishnendu Khan ; KD Validated