IBA1 Monoclonal antibody

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  IHC staining of human tonsillitis using 66827-2-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66827-2-Ig (IBA1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66827-2-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66827-2-Ig (IBA1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66827-2-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66827-2-Ig (IBA1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66827-2-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66827-2-Ig (IBA1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IBA1 antibodies are primarily used as markers for microglia, the resident immune cells of the central nervous system, and for macrophages in peripheral tissues. Proteintech has an extensive validated IBA1 antibodies for IHC, ELISA.

Cat No. 66827-2-Ig

Clone No.2B2A12

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

IF, IHC, ELISA

Synonyms

AIF 1, AIF1, G1, IBA1, IRT 1, Protein G1

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Tested Applications

• Positive IHC detected in: human brain tissue, human liver cancer tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

66827-2-Ig targets IBA1 in IF, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IBA1 fusion protein Ag28236
• Full Name: allograft inflammatory factor 1
• Calculated Molecular Weight: 17 kDa
• GenBank Accession Number: BC009474
• Gene Symbol: IBA1
• Gene ID (NCBI): 199
• ENSEMBL Gene ID: ENSG00000204472
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Antioxidants (Basel); TNF-α Levels Are Increased in Patients with Subjective Cognitive Impairment and Are Negatively Correlated with β Amyloid-42; Authors - Sara Serafini