ICAM-1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ICAM-1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, FC, CoIP, Blocking, ELISA

Conjugate

Unconjugated

Publications

136

Reviews (1)

Cat no : 10831-1-AP

Synonyms

BB2, CD54, ICAM 1, ICAM1, ICAM-1, P3.58

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Validation Data Gallery

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  WB analysis of Raji using 10831-1-AP

  WB result of ICAM1 antibody (10831-1-AP, 1:1000) with si-Control and si-ICAM1 transfected Raji cells..

• 

  WB analysis using 10831-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10831-1-AP (ICAM-1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 10831-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10831-1-AP (ICAM-1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 10831-1-AP

  IP Result of anti-ICAM-1 (IP:10831-1-AP, 4ug; Detection:10831-1-AP 1:1000) with Raji cells lysate 1320ug.

• 

  IHC staining of human lung cancer using 10831-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10831-1-AP (ICAM-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10831-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10831-1-AP (ICAM-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10831-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10831-1-AP (ICAM-1 antibody) at dilution of 1:50 (under 25x lens).

• 

  IHC staining of human tonsillitis using 10831-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10831-1-AP (ICAM-1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 10831-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10831-1-AP (ICAM-1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 10831-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10831-1-AP (ICAM-1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 10831-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10831-1-AP (ICAM-1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 10831-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10831-1-AP (ICAM-1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 10831-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10831-1-AP (ICAM-1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of human lung cancer using 10831-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using ICAM-1 antibody (10831-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human lung cancer using 10831-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using ICAM-1 antibody (10831-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Raji using 10831-1-AP

  1X10^6 Raji cells were stained with 0.2ug ICAM-1 antibody (10831-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: HUVEC cells, L02 cells, Raji cells
• Positive IP detected in: Raji cells
• Positive IHC detected in: human lung cancer tissue, human tonsillitis tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human lung cancer tissue
• Positive FC detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 109 publications below
• IHC: See 22 publications below
• IF: See 19 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

10831-1-AP targets ICAM-1 in WB, IP, IHC, IF, FC, CoIP, Blocking, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, bovine, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ICAM-1 fusion protein Ag1188
• Full Name: intercellular adhesion molecule 1
• Calculated molecular weight: 90 kDa
• Observed molecular weight: 85-95 kDa
• GenBank accession number: BC015969
• Gene symbol: ICAM1
• Gene ID (NCBI): 3383
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Where is ICAM-1 expressed?

Intercellular Adhesion Molecule 1 (ICAM-1), also known as Cluster of Differentiation 54 (CD54) is a transmembrane glycoprotein constitutively expressed at low levels in endothelial cells, pericytes and on some lymphocytes and monocytes¹. It is located at the cytoplasmic membrane, with a large extracellular region of mainly hydrophobic amino acids joined to a small transmembrane region and a cytoplasmic tail. It has a molecular weight of 75 to 115 kDa depending on the level of glycosylation.

 What is the function of ICAM-1?

ICAM-1 is important in both innate and adaptive immune responses as an adhesion molecule. Although it is constitutively expressed, in the presence of pro-inflammatory cytokines such as TNFα the endothelial cells are activated and upregulate expression of ICAM-1². In blood vessels lined with endothelial cells, leukocytes that are rolling over the surface are able to bind to ICAM-1 and transmigrate through the endothelial barrier and into the tissue. The initial binding of the leukocytes to ICAM-1 causes a Ca2+ release that initiates endothelial cell contraction and weakening of the intercellular tight junctions^{3, 4}. This protein can be used as an indicator of endothelial activation and of vascular inflammation.

 What is the role of ICAM-1 in disease?

Beyond the role in the immune response, ICAM-1 has also been identified as the target of attachment for the human rhinovirus, the cause of the common cold. Binding of the virus to ICAM-1 causes the viral capsid to uncoat and leads to release of the genetic material⁵.

1.  Hubbard, A. K. & Rothlein, R. Intercellular adhesion molecule-1 (ICAM-1) expression and cell signaling cascades. Free Radic. Biol. Med. 28, 1379-86 (2000).

2. Long, E. O. ICAM-1: getting a grip on leukocyte adhesion. J. Immunol. 186, 5021-3 (2011).

3. Lawson, C. & Wolf, S. ICAM-1 signaling in endothelial cells. (2009).

4. Lyck, R. & Enzmann, G. The physiological roles of ICAM-1 and ICAM-2 in neutrophil migration into tissues. Curr. Opin. Hematol. 22, 53-59 (2015).

5. Xing, L., Casasnovas, J. M. & Cheng, R. H. Structural analysis of human rhinovirus complexed with ICAM-1 reveals the dynamics of receptor-mediated virus uncoating. J. Virol. 77, 6101-7 (2003).

Publications

Species	Application	Title
mouse	Blocking	Adv Sci (Weinh); Engineered Cell-Derived Microparticles Bi2Se3/DOX@MPs for Imaging Guided Synergistic Photothermal/Low-Dose Chemotherapy of Cancer.; Authors - Dongdong Wang
mouse	WB	Hepatology; Dysregulation of the unfolded protein response in db/db mice with diet-induced steatohepatitis.; Authors - Rinella Mary E ME
mouse	WB	Nat Commun; Tumor exosome-based nanoparticles are efficient drug carriers for chemotherapy.; Authors - Tuying Yong
rat	WB	Theranostics; Ligand Activation of PPARγ by Ligustrazine Suppresses Pericyte Functions of Hepatic Stellate Cells via SMRT-Mediated Transrepression of HIF-1α.; Authors - Feng Zhang
human	WB	Theranostics; Mitochondrial ROS promote mitochondrial dysfunction and inflammation in ischemic acute kidney injury by disrupting TFAM-mediated mtDNA maintenance.; Authors - Meng Zhao
human	WB	J Control Release; Injectable extracellular vesicle-released self-assembling peptide nanofiber hydrogel as an enhanced cell-free therapy for tissue regeneration.; Authors - Yijie Zhou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Uthra (Verified Customer) (10-03-2022)

Good!!!

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:150
• Cell Tissue Type: Leukocytes

ICAM-1 Antibody Immunofluorescence validation (1:150 dilution) in Leukocytes (Cat no:10831-1-AP)

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