ID1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ID1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 18475-1-AP

Synonyms

Inhibitor of DNA binding 1, Inhibitor of differentiation 1, ID, DNA-binding protein inhibitor ID-1, Class B basic helix-loop-helix protein 24

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Validation Data Gallery

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  WB analysis of PC-3 using 18475-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18475-1-AP (ID1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 18475-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18475-1-AP (ID1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-3 using 18475-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using ID1 antibody (18475-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: PC-3 cells
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Product Information

18475-1-AP targets ID1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ID1 fusion protein Ag13359
• Full Name: inhibitor of DNA binding 1, dominant negative helix-loop-helix protein
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC000613
• Gene Symbol: ID1
• Gene ID (NCBI): 3397
• RRID: AB_2248812
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ID (inhibitor of DNA binding) proteins contain a helix-loop-helix (HLH) motif and regulate tissue-specific transcription within several cell lineages. They do not bind DNA directly, but inhibit lineage commitment by binding basic helix-loop-helix (bHLH) transcription factors through their HLH motif. ID1 proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID proteins contribute to cell growth, senescence, differentiation, and angiogenesis. Inhibitor of differentiation or DNA binding-1 (ID1) interacts with the transactivation domain of p65 (p65TAD) both in vitro and in vivo. The molecular weight of ID1 is about 20 kDa.

Publications

Species	Application	Title
human,mouse	WB,IF	Nat Commun; ID1 expressing macrophages support cancer cell stemness and limit CD8+ T cell infiltration in colorectal cancer; Authors - Shuang Shang
rat	WB	Basic Res Cardiol; The miR-182/Myadm axis regulates hypoxia-induced pulmonary hypertension by balancing the BMP- and TGF-β-signalling pathways in an SMC/EC-crosstalk-associated manner; Authors - Yongyi Bai
mouse	IF	EMBO J; Defining multistep cell fate decision pathways during pancreatic development at single-cell resolution.; Authors - Xin-Xin Yu
mouse	WB	Clin Transl Med; Ursodesoxycholic acid alleviates liver fibrosis via proregeneration by activation of the ID1-WNT2/HGF signaling pathway.; Authors - Xi Dong
human,mouse	WB	Environ Health Perspect; Exposure to ambient air fine particulate matter prevents VEGF-induced mobilization of endothelial progenitor cells from the bone marrow.; Authors - Haberzettl Petra P
human	WB	Acta Pharmacol Sin; Pantoprazole ameliorates liver fibrosis and suppresses hepatic stellate cell activation in bile duct ligation rats by promoting YAP degradation; Authors - Zhen-ning Lu