|Positive WB detected in||PC-3 cells|
|Positive IF detected in||PC-3 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
18475-1-AP targets ID1 in WB, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ID1 fusion protein Ag13359|
|Full Name||inhibitor of DNA binding 1, dominant negative helix-loop-helix protein|
|Calculated molecular weight||16 kDa|
|Observed molecular weight||20 kDa, 45-50 kDa|
|GenBank accession number||BC000613|
|Gene ID (NCBI)||3397|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ID (inhibitor of DNA binding) proteins contain a helix-loop-helix (HLH) motif and regulate tissue-specific transcription within several cell lineages. They do not bind DNA directly, but inhibit lineage commitment by binding basic helix-loop-helix (bHLH) transcription factors through their HLH motif. ID1 proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID proteins contribute to cell growth, senescence, differentiation, and angiogenesis. Inhibitor of differentiation or DNA binding-1 (ID1) interacts with the transactivation domain of p65 (p65TAD) both in vitro and in vivo. The molecular weight of ID1 and p65 complex is about 45-50 kDa (PMID: 22592405 ).
Exp Eye Res
Expansion and maintenance of primary corneal epithelial stem/progenitor cells by inhibition of TGFβ receptor I-mediated signaling.
Biochim Biophys Acta
BMP-6 inhibits cell proliferation by targeting microRNA-192 in breast cancer.
Id1/NR2B Receptor Pathway Regulates Rat Cochlear Sensory Epithelial Cell Survival after Radiation.
Int J Med Sci
IL-1β Promotes Stemness of Tumor Cells by Activating Smad/ID1 Signaling Pathway.
High extracellular pressure promotes gastric cancer cell adhesion, invasion, migration and suppresses gastric cancer cell differentiation.
J Neurosci Res
Bone morphogenetic protein 4, inhibitor of differentiation 1, and epidermal growth factor receptor regulate the survival of cochlear sensory epithelial cells.