- Featured Product
- KD/KO Validated
ID1 Polyclonal antibody
ID1 Polyclonal Antibody for IF, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IHC, IF, ELISA
Cat no : 18475-1-AP
|Positive WB detected in||PC-3 cells|
|Positive IF detected in||PC-3 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 12 publications below|
|IHC||See 1 publications below|
|IF||See 4 publications below|
18475-1-AP targets ID1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ID1 fusion protein Ag13359|
|Full Name||inhibitor of DNA binding 1, dominant negative helix-loop-helix protein|
|Calculated molecular weight||16 kDa|
|Observed molecular weight||20 kDa|
|GenBank accession number||BC000613|
|Gene ID (NCBI)||3397|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
ID (inhibitor of DNA binding) proteins contain a helix-loop-helix (HLH) motif and regulate tissue-specific transcription within several cell lineages. They do not bind DNA directly, but inhibit lineage commitment by binding basic helix-loop-helix (bHLH) transcription factors through their HLH motif. ID1 proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID proteins contribute to cell growth, senescence, differentiation, and angiogenesis. Inhibitor of differentiation or DNA binding-1 (ID1) interacts with the transactivation domain of p65 (p65TAD) both in vitro and in vivo. The molecular weight of ID1 is about 20 kDa.
|Product Specific Protocols|
|WB protocol for ID1 antibody 18475-1-AP||Download protocol|
|IF protocol for ID1 antibody 18475-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Basic Res Cardiol
The miR-182/Myadm axis regulates hypoxia-induced pulmonary hypertension by balancing the BMP- and TGF-β-signalling pathways in an SMC/EC-crosstalk-associated manner
Defining multistep cell fate decision pathways during pancreatic development at single-cell resolution.
Clin Transl Med
Ursodesoxycholic acid alleviates liver fibrosis via proregeneration by activation of the ID1-WNT2/HGF signaling pathway.
Environ Health Perspect
Exposure to ambient air fine particulate matter prevents VEGF-induced mobilization of endothelial progenitor cells from the bone marrow.
Ecotoxicol Environ Saf
Exosomal miR-125a-5p derived from silica-exposed macrophages induces fibroblast transdifferentiation.
Am J Physiol Heart Circ Physiol
Endothelial progenitor cells as critical mediators of environmental air pollution-induced cardiovascular toxicity.