|Positive WB detected in||HEK-293 cells, rat liver tissue, HeLa cells, HepG2 cells, NIH/3T3 cells|
|Positive IP detected in||MCF-7 cells|
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
12332-1-AP targets IDH1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IDH1 fusion protein Ag2986|
|Full Name||isocitrate dehydrogenase 1 (NADP+), soluble|
|Calculated molecular weight||414 aa, 47 kDa|
|Observed molecular weight||47 kDa|
|GenBank accession number||BC012846|
|Gene ID (NCBI)||3417|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171). IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ).
|Product Specific Protocols|
|WB protocol for IDH1 antibody 12332-1-AP||Download protocol|
|IF protocol for IDH1 antibody 12332-1-AP||Download protocol|
|IP protocol for IDH1 antibody 12332-1-AP||Download protocol|
|FC protocol for IDH1 antibody 12332-1-AP||Download protocol|
|Click here to view our Standard Protocols|
α-Ketoglutarate links p53 to cell fate during tumour suppression.
IDH mutation impairs histone demethylation and results in a block to cell differentiation.
Cancer-associated IDH1 mutations produce 2-hydroxyglutarate.
Quantitative flux analysis reveals folate-dependent NADPH production.
Leukemic IDH1 and IDH2 mutations result in a hypermethylation phenotype, disrupt TET2 function, and impair hematopoietic differentiation.
Cell Stem Cell
PHB Associates with the HIRA Complex to Control an Epigenetic-Metabolic Circuit in Human ESCs.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Umut (Verified Customer) (07-25-2023)
The antibody worked very well for Western Blot in a standard application with an average dilution and exposure.