IFIT1 Antibody

IFIT1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 23247-1-AP

Synonyms

G10P1, GARG 16, IFI 56, IFI 56K, IFI56, IFIT 1, IFIT1, IFNAI1, ISG56, RNM561



Tested Applications

Positive WB detected inU-937 cells, HeLa cells, LPS treated RAW 264.7 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The 23247-1-AP immunogen is Fusion Protein IFIT1 protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFIT1 fusion protein Ag19691
Full Name interferon-induced protein with tetratricopeptide repeats 1
Calculated molecular weight 478aa,55 kDa
Observed molecular weight 55 Kda
GenBank accession number BC007091
Gene symbol IFIT1
Gene ID (NCBI) 3434
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFIT1 is, also known as glucocorticoid-attenuated response gene 16 protein (GARG-16), is a 463 amino acid protein belonging to the IFIT family. IFIT genes comprise a large family with three (Ifit1, Ifit2, and Ifit3) and four (IFIT1, IFIT2, IFIT3, and IFIT5) members in mice and humans, respectively. IFIT1 specifically bound virus PPP-RNA forms a complex with IFIT2 and IFIT3 to sequester the viral PPP-RNA and prevent virus replication.

Protocols

Product Specific Protocols
WB protocol for IFIT1 antibody 23247-1-APDownload protocol
IHC protocol for IFIT1 antibody 23247-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Life Sci

Mechanism of berberine in treating Helicobacter pylori induced chronic atrophic gastritis through IRF8-IFN-γ signaling axis suppressing.

Authors - Tao Yang
humanWB

J Proteome Res

Label-free Proteomic Analysis of Exosomes Secreted from THP-1-derived Macrophages Treated with IFN-α Identifies Antiviral Proteins Enriched in Exosomes.

Authors - Zhenlan Yao
humanWB

J Biol Chem

A RIG-I-like receptor directs antiviral responses to a bunyavirus and is antagonized by virus-induced blockade of TRIM25-mediated ubiquitination.

Authors - Yuan-Qin Min
humanIF

J Virol

Suppression of Stromal Interferon Signaling by Human Papillomavirus 16.

Authors - Gaurav Raikhy
humanWB

J Virol

Human papillomavirus type 16 E5 inhibits interferon signaling and supports episomal viral maintenance.

Authors - Matthew L Scott
WB

J Virol

Herpes Simplex Virus 1 Tegument Protein UL41 Counteracts IFIT3 Antiviral Innate Immunity.

Authors - Zhangtao Jiang