IFIT2 Polyclonal antibody

• Featured Product
• KD/KO Validated

IFIT2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

31

Cat no : 12604-1-AP

Synonyms

cig42, G10P2, GARG 39, IFI 54, IFI 54K, IFI54, IFIT 2, IFIT2, ISG 54 K, ISG 54K, ISG54

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Validation Data Gallery

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  WB analysis of A431 cells using 12604-1-AP

  WB result of IFIT2 antibody (12604-1-AP, 1:300) with si-IFIT2 and si-Control transfected A431 cells.

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  WB analysis of A431 using 12604-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12604-1-AP (IFIT2 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of RAW 264.7 using 12604-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 12604-1-AP (IFIT2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skin using 12604-1-AP

  IP Result of anti-IFIT2 (IP:12604-1-AP, 4ug; Detection:12604-1-AP 1:300) with mouse skin tissue lysate 3200ug.

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  IHC staining of human skin cancer using 12604-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12604-1-AP (IFIT2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 12604-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12604-1-AP (IFIT2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 12604-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12604-1-AP (IFIT2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 12604-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12604-1-AP (IFIT2 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 12604-1-AP

  Immunofluorescent analysis of HepG2 cells using 12604-1-AP (IFIT2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, RAW 264.7 cells
• Positive IP detected in: mouse skin tissue
• Positive IHC detected in: human skin cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 29 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

12604-1-AP targets IFIT2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFIT2 fusion protein Ag3283
• Full Name: IFIT 2
• Calculated molecular weight: 484 aa, 56 kDa
• Observed molecular weight: 50-55 kDa
• GenBank accession number: BC032839
• Gene symbol: IFIT2
• Gene ID (NCBI): 3433
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IFIT2 belongs to the IFIT family. It contains 6 TPR repeats. IFIT genes comprise a large family with three (Ifit1, Ifit2, and Ifit3) and four (IFIT1, IFIT2, IFIT3, and IFIT5) members in mice and humans, respectively. IFIT proteins are produced in human body that are supposed to confer immunity against viral infection. These proteins are generally produced during viral infection, IFN treatment, and during pathogen recognition (Pathogen associated molecular pattern recognition) by immune system during infections.

Publications

Species	Application	Title
human	WB	PLoS Pathog; The histone chaperone HIRA promotes the induction of host innate immune defences in response to HSV-1 infection.; Authors - Steven McFarlane
	WB	Wellcome Open Res; PMID: 31372503; Authors - Harriet V Mears
human	WB	Cell Rep; Cell Cycle Checkpoints Cooperate to Suppress DNA- and RNA-Associated Molecular Pattern Recognition and Anti-Tumor Immune Responses.; Authors - Jie Chen
human	WB	bioRxiv; ISG15-dependent Activation of the RNA Sensor MDA5 and its Antagonism by the SARS-CoV-2 papain-like protease.; Authors - GuanQun Liu
human	WB	mBio; The Small t Antigen of JC Virus Antagonizes RIG-I-Mediated Innate Immunity by Inhibiting TRIM25's RNA Binding Ability.; Authors - Cindy Chiang
human	WB	mBio; Human IFIT3 Protein Induces Interferon Signaling and Inhibits Adenovirus Immediate Early Gene Expression.; Authors - Aniska Chikhalya

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