IFNAR1 Polyclonal antibody

IFNAR1 Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 13083-1-AP

Synonyms

AVP, CRF2 1, IFN alpha REC, IFN alpha/beta receptor 1, IFN R 1, IFNAR, IFNAR1, IFNBR, IFRC, Type I interferon receptor 1



Tested Applications

Positive WB detected inJurkat cells, K-562 cell, mouse liver tissue
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

13083-1-AP targets IFNAR1 in WB, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFNAR1 fusion protein Ag3739
Full Name interferon (alpha, beta and omega) receptor 1
Calculated molecular weight 557 aa, 64 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC021825
Gene symbol IFNAR1
Gene ID (NCBI) 3454
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interferon alpha and beta receptor subunit 1 (IFNAR1) is a type I membrane protein that associates with IFNAR2 to form the receptor for type I interferons, including interferons-alpha, -beta, and -lambda. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2, and then increase transcription of the IFN-induced genes whose products exert antiviral, immunomodulatory, and antiproliferative effects.

Protocols

Product Specific Protocols
WB protocol for IFNAR1 antibody 13083-1-APDownload protocol
FC protocol for IFNAR1 antibody 13083-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

TDP-43 prevents endogenous RNAs from triggering a lethal RIG-I-dependent interferon response.

Authors - William Dunker
humanWB

Food Nutr Res

PMID: 27356910

Authors - Sheng Li
humanWB,IF

Oncotarget

PMID: 26683360

Authors - Yujiao He
humanWB

Elife

The oncoprotein BCL6 enables solid tumor cells to evade genotoxic stress.

Authors - Yanan Liu
  • KD Validated
humanWB

EMBO Rep

Degradation of WTAP blocks antiviral responses by reducing the m 6 A levels of IRF3 and IFNAR1 mRNA

Authors - Yong Ge

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Christine (Verified Customer) (07-12-2022)

Both cell lines are human ones, only the RPE1 cells express the IFNAR1 receptor. IFNAR1 is described in the literature as heavily glycosylated and hence its molecular weight can vary from 64-135 kDa depending on its glycosylation level. We generally observed in RPE1 cells fully matured 110-130 kDa major band on western blot, which is not observed at all here with the 13083-1-AP antibody. The 72 kDa bands seems to be present only in RPE1 and not in the IFNAR1 null Huh7 cells, indicating it could indeed be immature non glycosylated forms of IFNAR1. (We also observed these minor bands on RPE1 cells with another IFNAR1 antibody , but at a much lower intensity than the 110 kDa one)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: hTert-RPE1 cells and Huh7 cells (IFNAR1 null cells)
IFNAR1 Antibody Western Blot validation (1/1000 dilution) in hTert-RPE1 cells and Huh7 cells (IFNAR1 null cells) (Cat no:13083-1-AP)