- Featured Product
- KD/KO Validated
IFT52 Polyclonal antibody
IFT52 Polyclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat, Canine and More (1)
WB, IP, IF, CoIP, ELISA
Cat no : 17534-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, human testis tissue, mouse testis tissue, MDCK cells, LNCaP cells|
|Positive IP detected in||mouse testis tissue, HeLa cells|
|Positive IF detected in||Hela cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 2 publications below|
|WB||See 13 publications below|
|IF||See 7 publications below|
|IP||See 1 publications below|
|CoIP||See 1 publications below|
17534-1-AP targets IFT52 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.
|Tested Reactivity||human, mouse, rat, Canine|
|Cited Reactivity||human, mouse, Chicken|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IFT52 fusion protein Ag11586|
|Full Name||intraflagellar transport 52 homolog (Chlamydomonas)|
|Calculated molecular weight||437 aa, 50 kDa|
|Observed molecular weight||45-50 kDa|
|GenBank accession number||BC039831|
|Gene ID (NCBI)||51098|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
IFT52, also named as C20orf9 and NGD5, is a component of IFT complex B which is composed of IFT88, IFT57, TRAF3IP1, IFT52, IFT27, HSPB11 and IFT20. IFT52 binds directly to the IFT81/74/27/25 complex. And IFT52 could mediate the interaction between the two subcomplexes.(PMID:21642430) The MW of IFT52 is about 45-54kd.
|Product Specific Protocols|
|WB protocol for IFT52 antibody 17534-1-AP||Download protocol|
|IF protocol for IFT52 antibody 17534-1-AP||Download protocol|
|IP protocol for IFT52 antibody 17534-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Nat Cell Biol
The cilia protein IFT88 is required for spindle orientation in mitosis.
IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning.
Am J Hum Genet
Bi-allelic Mutations in TTC29 Cause Male Subfertility with Asthenoteratospermia in Humans and Mice.
Am J Hum Genet
Bi-allelic Mutations in TTC21A Induce Asthenoteratospermia in Humans and Mice.
Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.
Proc Natl Acad Sci U S A
FGFR1-mediated protocadherin-15 loading mediates cargo specificity during intraflagellar transport in inner ear hair-cell kinocilia.