IFT52 Polyclonal antibody

• Featured Product
• KD/KO Validated

IFT52 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine and More (1)

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

20

Cat no : 17534-1-AP

Synonyms

C20orf9, CGI 53, IFT52, NGD5, Protein NGD5 homolog

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Validation Data Gallery

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  WB analysis using 17534-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17534-1-AP (IFT52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 17534-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 17534-1-AP (IFT52 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 17534-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17534-1-AP (IFT52 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MDCK using 17534-1-AP

  MDCK cells were subjected to SDS PAGE followed by western blot with 17534-1-AP (IFT52 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 17534-1-AP

  IP Result of anti-IFT52 (IP:17534-1-AP, 4ug; Detection:17534-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

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  IP experiment of HeLa cells using 17534-1-AP

  IP result of anti-IFT52 (17534-1-AP for IP and Detection) with HeLa cell lysate.

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  IF Staining of HeLa using 17534-1-AP

  Immunofluorescent analysis of Hela cells, using IFT52 antibody 17534-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, human testis tissue, mouse testis tissue, MDCK cells, LNCaP cells
• Positive IP detected in: mouse testis tissue, HeLa cells
• Positive IF detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

17534-1-AP targets IFT52 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.

• Tested Reactivity: human, mouse, rat, Canine
• Cited Reactivity: human, mouse, Chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFT52 fusion protein Ag11586
• Full Name: intraflagellar transport 52 homolog (Chlamydomonas)
• Calculated molecular weight: 437 aa, 50 kDa
• Observed molecular weight: 45-50 kDa
• GenBank accession number: BC039831
• Gene symbol: IFT52
• Gene ID (NCBI): 51098
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFT52, also named as C20orf9 and NGD5, is a component of IFT complex B which is composed of IFT88, IFT57, TRAF3IP1, IFT52, IFT27, HSPB11 and IFT20. IFT52 binds directly to the IFT81/74/27/25 complex. And IFT52 could mediate the interaction between the two subcomplexes.(PMID:21642430) The MW of IFT52 is about 45-54kd.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; The cilia protein IFT88 is required for spindle orientation in mitosis.; Authors - Delaval Benedicte B
human	WB	Nat Commun; IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning.; Authors - Nicolas Taulet
human	WB	Am J Hum Genet; Bi-allelic Mutations in TTC29 Cause Male Subfertility with Asthenoteratospermia in Humans and Mice.; Authors - Chunyu Liu
mouse	CoIP	Am J Hum Genet; Bi-allelic Mutations in TTC21A Induce Asthenoteratospermia in Humans and Mice.; Authors - Wangjie Liu
mouse	WB	EMBO J; Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.; Authors - Shichao Duan
Chicken	IP	Proc Natl Acad Sci U S A; FGFR1-mediated protocadherin-15 loading mediates cargo specificity during intraflagellar transport in inner ear hair-cell kinocilia.; Authors - Akira Honda