- Featured Product
- KD/KO Validated
IFT52 Polyclonal antibody
IFT52 Polyclonal Antibody for IF, IP, WB, ELISA
Cat no : 17534-1-AP
Validation Data Gallery
|Positive WB detected in
|HEK-293 cells, human testis tissue, mouse testis tissue, MDCK cells, LNCaP cells
|Positive IP detected in
|mouse testis tissue, HeLa cells
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:1000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IF : 1:10-1:100
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
17534-1-AP targets IFT52 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.
|human, mouse, rat, Canine
|human, mouse, rat, Chicken
|Host / Isotype
|Rabbit / IgG
|IFT52 fusion protein Ag11586
|intraflagellar transport 52 homolog (Chlamydomonas)
|Calculated molecular weight
|437 aa, 50 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
IFT52, also named as C20orf9 and NGD5, is a component of IFT complex B which is composed of IFT88, IFT57, TRAF3IP1, IFT52, IFT27, HSPB11 and IFT20. IFT52 binds directly to the IFT81/74/27/25 complex. And IFT52 could mediate the interaction between the two subcomplexes.(PMID:21642430) The MW of IFT52 is about 45-54kd.
Nat Cell Biol
The cilia protein IFT88 is required for spindle orientation in mitosis.
IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning.
Am J Hum Genet
Bi-allelic Mutations in TTC29 Cause Male Subfertility with Asthenoteratospermia in Humans and Mice.
Am J Hum Genet
Bi-allelic Mutations in TTC21A Induce Asthenoteratospermia in Humans and Mice.
Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.
Proc Natl Acad Sci U S A
FGFR1-mediated protocadherin-15 loading mediates cargo specificity during intraflagellar transport in inner ear hair-cell kinocilia.