Tested Applications
| Positive WB detected in | HEK-293 cells, human testis tissue, mouse testis tissue, MDCK cells, LNCaP cells |
| Positive IP detected in | mouse testis tissue, HeLa cells |
| Positive IF/ICC detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17534-1-AP targets IFT52 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
| Tested Reactivity | human, mouse, rat, canine |
| Cited Reactivity | human, mouse, rat, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11586 Product name: Recombinant human IFT52 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-437 aa of BC039831 Sequence: MEKELRSTILFNAYKKEIFTTNNGYKSMQKKLRSNWKIQSLKDEITSEKLNGVKLWITAGPREKFTAAEFEILKKYLDTGGDVFVMLGEGGESRFDTNINFLLEEYGIMVNNDAVVRNVYHKYFHPKEALVSSGVLNREISRAAGKAVPGIIDEESSGNNAQALTFVYPFGATLSVMKPAVAVLSTGSVCFPLNRPILAFYHSKNQGGKLAVLGSCHMFSDQYLDKEENSKIMDVVFQWLTTGDIHLNQIDAEDPEISDYMMLPYTATLSKRNRECLQESDEIPRDFTTLFDLSIFQLDTTSFHSVIEAHEQLNVKHEPLQLIQPQFETPLPTLQPAVFPPSFRELPPPPLELFDLDETFSSEKARLAQITNKCTEEDLEFYVRKCGDILGVTSKLPKDQQDAKHILEHVFFQVVEFKKLNQEHDIDTSETAFQNNF Predict reactive species |
| Full Name | intraflagellar transport 52 homolog (Chlamydomonas) |
| Calculated Molecular Weight | 437 aa, 50 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | BC039831 |
| Gene Symbol | IFT52 |
| Gene ID (NCBI) | 51098 |
| RRID | AB_2280005 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y366 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IFT52, also named as C20orf9 and NGD5, is a component of IFT complex B which is composed of IFT88, IFT57, TRAF3IP1, IFT52, IFT27, HSPB11 and IFT20. IFT52 binds directly to the IFT81/74/27/25 complex. And IFT52 could mediate the interaction between the two subcomplexes.(PMID:21642430) The MW of IFT52 is about 45-54kd.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IFT52 antibody 17534-1-AP | Download protocol |
| IP protocol for IFT52 antibody 17534-1-AP | Download protocol |
| WB protocol for IFT52 antibody 17534-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IFT52 Polyclonal antibody (Cat# 17534-1-AP) has 27 citations across journals including Nature Cell Biology, Nature Communications, PNAS, EMBO Journal, American Journal of Human Genetics, Human Molecular Genetics, Journal of the American Society of Nephrolology, Science Reports, FASEB Journal, and others. Research fields span intraflagellar transport, ciliogenesis, ciliopathies (polycystic kidney disease, Bardet-Biedl syndrome, short-rib polydactyly), Hedgehog signaling, centrosome biology, male infertility, and microtubule dynamics.
| Species | Application | Title |
|---|---|---|
Nat Commun IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning. | ||
J Am Soc Nephrol KLC3 Regulates Ciliary Trafficking and Cyst Progression in CILK1 Deficiency-Related Polycystic Kidney Disease | ||
Proc Natl Acad Sci U S A FGFR1-mediated protocadherin-15 loading mediates cargo specificity during intraflagellar transport in inner ear hair-cell kinocilia. | ||
EMBO J Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling. | ||
Am J Hum Genet Bi-allelic Mutations in TTC29 Cause Male Subfertility with Asthenoteratospermia in Humans and Mice. |
Reviews
What customers say
The single review for this antibody (rated 3/5) describes an immunofluorescence experiment in LIGHT2 cells using a standard protocol (1/200 dilution, overnight at 4°C). The reviewer found the signal pattern very "dotty," making it difficult to distinguish true IFT52 localization from background. They suggested the protocol may need optimization for this specific antibody, or that it might perform better in alternative cell lines. Overall, the feedback indicates the antibody requires further protocol tuning for clear, interpretable results in IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Charlotte (Verified Customer) (07-22-2024) | I tested the antibody following the classic iF protocol: PFA 4% 10 min cold methanol 5 min 1% BSA in PBST 1/200 in blocking solution, O.N. at 4°C 5*5min PBST 1/500 in blocking solution, 1h at RT° 4*5min PBST + 5min PBS However, the result is very doty which makes it hard to interpret and to distinguish IFT52 real signal (signal should be similar to the ARL13B one or as dots along a line if not showing a continuous line). It could be due to the protocol that needs to be adjusted for this antibody or maybe the antibody works better in other cell lines
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