IFT52 Polyclonal antibody

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  WB analysis using 17534-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17534-1-AP (IFT52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 17534-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 17534-1-AP (IFT52 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 17534-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17534-1-AP (IFT52 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MDCK using 17534-1-AP

  MDCK cells were subjected to SDS PAGE followed by western blot with 17534-1-AP (IFT52 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 17534-1-AP

  IP result of anti-IFT52 (IP:17534-1-AP, 4ug; Detection:17534-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

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  IP experiment of HeLa cells using 17534-1-AP

  IP result of anti-IFT52 (17534-1-AP for IP and Detection) with HeLa cell lysate.

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  IF Staining of HeLa using 17534-1-AP

  Immunofluorescent analysis of Hela cells, using IFT52 antibody 17534-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• Featured Product
• KD/KO Validated

IFT52 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 17534-1-AP

Publications(24)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine and More (1)

Applications

WB, IF/ICC, IP, CoIP, ELISA

Synonyms

NGD5, IFT, CGI-53, C20orf9

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Tested Applications

• Positive WB detected in: HEK-293 cells, human testis tissue, mouse testis tissue, MDCK cells, LNCaP cells
• Positive IP detected in: mouse testis tissue, HeLa cells
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 17 publications below
• IF: See 9 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

17534-1-AP targets IFT52 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

• Tested Reactivity: human, mouse, rat, canine
• Cited Reactivity: human, mouse, rat, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFT52 fusion protein Ag11586
• Full Name: intraflagellar transport 52 homolog (Chlamydomonas)
• Calculated Molecular Weight: 437 aa, 50 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC039831
• Gene Symbol: IFT52
• Gene ID (NCBI): 51098
• RRID: AB_2280005
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y366
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFT52, also named as C20orf9 and NGD5, is a component of IFT complex B which is composed of IFT88, IFT57, TRAF3IP1, IFT52, IFT27, HSPB11 and IFT20. IFT52 binds directly to the IFT81/74/27/25 complex. And IFT52 could mediate the interaction between the two subcomplexes.(PMID:21642430) The MW of IFT52 is about 45-54kd.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; The cilia protein IFT88 is required for spindle orientation in mitosis.; Authors - Delaval Benedicte B
human	WB	Nat Commun; IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning.; Authors - Nicolas Taulet
human	WB	Am J Hum Genet; Bi-allelic Mutations in TTC29 Cause Male Subfertility with Asthenoteratospermia in Humans and Mice.; Authors - Chunyu Liu
mouse	CoIP	Am J Hum Genet; Bi-allelic Mutations in TTC21A Induce Asthenoteratospermia in Humans and Mice.; Authors - Wangjie Liu
mouse	WB	EMBO J; Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.; Authors - Shichao Duan
Chicken	IP	Proc Natl Acad Sci U S A; FGFR1-mediated protocadherin-15 loading mediates cargo specificity during intraflagellar transport in inner ear hair-cell kinocilia.; Authors - Akira Honda

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Charlotte (Verified Customer) (07-22-2024)

I tested the antibody following the classic iF protocol: PFA 4% 10 min cold methanol 5 min 1% BSA in PBST 1/200 in blocking solution, O.N. at 4°C 5*5min PBST 1/500 in blocking solution, 1h at RT° 4*5min PBST + 5min PBS However, the result is very doty which makes it hard to interpret and to distinguish IFT52 real signal (signal should be similar to the ARL13B one or as dots along a line if not showing a continuous line). It could be due to the protocol that needs to be adjusted for this antibody or maybe the antibody works better in other cell lines

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/200
• Cell Tissue Type: LIGHT2

IFT52 Antibody Immunofluorescence validation (1/200 dilution) in LIGHT2 (Cat no:17534-1-AP)