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IFT52 Polyclonal antibody

IFT52 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine and More (1)

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 17534-1-AP

Synonyms

C20orf9, CGI 53, IFT52, NGD5, Protein NGD5 homolog



Tested Applications

Positive WB detected inHEK-293 cells, human testis tissue, mouse testis tissue, MDCK cells, LNCaP cells
Positive IP detected inmouse testis tissue, HeLa cells
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

17534-1-AP targets IFT52 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.

Tested Reactivity human, mouse, rat, Canine
Cited Reactivityhuman, mouse, rat, Chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFT52 fusion protein Ag11586
Full Name intraflagellar transport 52 homolog (Chlamydomonas)
Calculated molecular weight 437 aa, 50 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC039831
Gene symbol IFT52
Gene ID (NCBI) 51098
RRIDAB_2280005
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFT52, also named as C20orf9 and NGD5, is a component of IFT complex B which is composed of IFT88, IFT57, TRAF3IP1, IFT52, IFT27, HSPB11 and IFT20. IFT52 binds directly to the IFT81/74/27/25 complex. And IFT52 could mediate the interaction between the two subcomplexes.(PMID:21642430) The MW of IFT52 is about 45-54kd.

Protocols

Product Specific Protocols
WB protocol for IFT52 antibody 17534-1-APDownload protocol
IF protocol for IFT52 antibody 17534-1-APDownload protocol
IP protocol for IFT52 antibody 17534-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cell Biol

The cilia protein IFT88 is required for spindle orientation in mitosis.

Authors - Delaval Benedicte B
humanWB

Nat Commun

IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning.

Authors - Nicolas Taulet
humanWB

Am J Hum Genet

Bi-allelic Mutations in TTC29 Cause Male Subfertility with Asthenoteratospermia in Humans and Mice.

Authors - Chunyu Liu
mouseCoIP

Am J Hum Genet

Bi-allelic Mutations in TTC21A Induce Asthenoteratospermia in Humans and Mice.

Authors - Wangjie Liu
mouseWB

EMBO J

Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.

Authors - Shichao Duan
ChickenIP

Proc Natl Acad Sci U S A

FGFR1-mediated protocadherin-15 loading mediates cargo specificity during intraflagellar transport in inner ear hair-cell kinocilia.

Authors - Akira Honda