Validation Data Gallery
|Positive WB detected in||HEK-293 cells, human testis tissue, MDCK cells, mouse testis tissue|
|Positive IP detected in||mouse testis tissue, HeLa cells|
|Positive IF detected in||Hela cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
17534-1-AP targets IFT52 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.
|Tested Reactivity||human, mouse, rat, Canine|
|Cited Reactivity||human, mouse, Chicken|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IFT52 fusion protein Ag11586|
|Full Name||intraflagellar transport 52 homolog (Chlamydomonas)|
|Calculated molecular weight||437 aa, 50 kDa|
|Observed molecular weight||45-50 kDa|
|GenBank accession number||BC039831|
|Gene ID (NCBI)||51098|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
IFT52, also named as C20orf9 and NGD5, is a component of IFT complex B which is composed of IFT88, IFT57, TRAF3IP1, IFT52, IFT27, HSPB11 and IFT20. IFT52 binds directly to the IFT81/74/27/25 complex. And IFT52 could mediate the interaction between the two subcomplexes.(PMID:21642430) The MW of IFT52 is about 45-54kd.
Hum Mol Genet
Human IFT52 mutations uncover a novel role for the protein in microtubule dynamics and centrosome cohesion.
Robust interaction of IFT70 with IFT52-IFT88 in the IFT-B complex is required for ciliogenesis.
Characterization of tetratricopeptide repeat-containing proteins critical for cilia formation and function.
Inhibition of Hedgehog signaling suppresses proliferation and microcyst formation of human Autosomal Dominant Polycystic Kidney Disease cells.
J Med Genet
Homozygous mutations in CCDC34 cause male infertility with oligoasthenoteratozoospermia in humans and mice.
Destabilization of the IFT-B cilia core complex due to mutations in IFT81 causes a Spectrum of Short-Rib Polydactyly Syndrome.