IFT52 Polyclonal antibody

IFT52 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine and More (1)

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 17534-1-AP

Synonyms

C20orf9, CGI 53, IFT52, NGD5, Protein NGD5 homolog

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Validation Data Gallery

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  WB analysis using 17534-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17534-1-AP (IFT52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 17534-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 17534-1-AP (IFT52 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 17534-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17534-1-AP (IFT52 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MDCK using 17534-1-AP

  MDCK cells were subjected to SDS PAGE followed by western blot with 17534-1-AP (IFT52 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 17534-1-AP

  IP Result of anti-IFT52 (IP:17534-1-AP, 4ug; Detection:17534-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

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  IP experiment of HeLa cells using 17534-1-AP

  IP result of anti-IFT52 (17534-1-AP for IP and Detection) with HeLa cell lysate.

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  IF Staining of HeLa using 17534-1-AP

  Immunofluorescent analysis of Hela cells, using IFT52 antibody 17534-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, human testis tissue, MDCK cells, mouse testis tissue
• Positive IP detected in: mouse testis tissue, HeLa cells
• Positive IF detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IF: See 5 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

17534-1-AP targets IFT52 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.

• Tested Reactivity: human, mouse, rat, Canine
• Cited Reactivity: human, mouse, Chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFT52 fusion protein Ag11586
• Full Name: intraflagellar transport 52 homolog (Chlamydomonas)
• Calculated molecular weight: 437 aa, 50 kDa
• Observed molecular weight: 45-50 kDa
• GenBank accession number: BC039831
• Gene symbol: IFT52
• Gene ID (NCBI): 51098
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

IFT52, also named as C20orf9 and NGD5, is a component of IFT complex B which is composed of IFT88, IFT57, TRAF3IP1, IFT52, IFT27, HSPB11 and IFT20. IFT52 binds directly to the IFT81/74/27/25 complex. And IFT52 could mediate the interaction between the two subcomplexes.(PMID:21642430) The MW of IFT52 is about 45-54kd.

Publications

Species	Application	Title
human	WB,IF	Hum Mol Genet; Human IFT52 mutations uncover a novel role for the protein in microtubule dynamics and centrosome cohesion.; Authors - Marie Alice Dupont
human	WB	Biol Open; Robust interaction of IFT70 with IFT52-IFT88 in the IFT-B complex is required for ciliogenesis.; Authors - Ryota Takei
human	WB	PLoS One; Characterization of tetratricopeptide repeat-containing proteins critical for cilia formation and function.; Authors - Yanan Xu
human	IF	Sci Rep; Inhibition of Hedgehog signaling suppresses proliferation and microcyst formation of human Autosomal Dominant Polycystic Kidney Disease cells.; Authors - Luciane M Silva
human	IF	J Med Genet; Homozygous mutations in CCDC34 cause male infertility with oligoasthenoteratozoospermia in humans and mice.; Authors - Jiangshan Cong
human	WB	Sci Rep; Destabilization of the IFT-B cilia core complex due to mutations in IFT81 causes a Spectrum of Short-Rib Polydactyly Syndrome.; Authors - Ivan Duran