IFT52 Polyclonal antibody
IFT52 Polyclonal Antibody for IF, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, Canine and More (1)
Applications
WB, IP, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 17534-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, human testis tissue, MDCK cells, mouse testis tissue |
Positive IP detected in | mouse testis tissue, HeLa cells |
Positive IF detected in | Hela cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 10 publications below |
IF | See 5 publications below |
IP | See 1 publications below |
CoIP | See 1 publications below |
Product Information
17534-1-AP targets IFT52 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.
Tested Reactivity | human, mouse, rat, Canine |
Cited Reactivity | human, mouse, Chicken |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | IFT52 fusion protein Ag11586 |
Full Name | intraflagellar transport 52 homolog (Chlamydomonas) |
Calculated molecular weight | 437 aa, 50 kDa |
Observed molecular weight | 45-50 kDa |
GenBank accession number | BC039831 |
Gene symbol | IFT52 |
Gene ID (NCBI) | 51098 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IFT52, also named as C20orf9 and NGD5, is a component of IFT complex B which is composed of IFT88, IFT57, TRAF3IP1, IFT52, IFT27, HSPB11 and IFT20. IFT52 binds directly to the IFT81/74/27/25 complex. And IFT52 could mediate the interaction between the two subcomplexes.(PMID:21642430) The MW of IFT52 is about 45-54kd.
Protocols
Product Specific Protocols | |
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WB protocol for IFT52 antibody 17534-1-AP | Download protocol |
IF protocol for IFT52 antibody 17534-1-AP | Download protocol |
IP protocol for IFT52 antibody 17534-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Hum Mol Genet Human IFT52 mutations uncover a novel role for the protein in microtubule dynamics and centrosome cohesion. | ||
Biol Open Robust interaction of IFT70 with IFT52-IFT88 in the IFT-B complex is required for ciliogenesis. | ||
PLoS One Characterization of tetratricopeptide repeat-containing proteins critical for cilia formation and function. | ||
Sci Rep Inhibition of Hedgehog signaling suppresses proliferation and microcyst formation of human Autosomal Dominant Polycystic Kidney Disease cells. | ||
J Med Genet Homozygous mutations in CCDC34 cause male infertility with oligoasthenoteratozoospermia in humans and mice. | ||
Sci Rep Destabilization of the IFT-B cilia core complex due to mutations in IFT81 causes a Spectrum of Short-Rib Polydactyly Syndrome. |