IFT52 Polyclonal antibody

IFT52 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine and More (1)

Applications

WB, IP, IF, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 17534-1-AP

Synonyms

C20orf9, CGI 53, IFT52, NGD5, Protein NGD5 homolog



Tested Applications

Positive WB detected inHEK-293 cells, human testis tissue, MDCK cells, mouse testis tissue
Positive IP detected inmouse testis tissue, HeLa cells
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

17534-1-AP targets IFT52 in WB, IP, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat, Canine samples.

Tested Reactivity human, mouse, rat, Canine
Cited Reactivity Chicken, human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFT52 fusion protein Ag11586
Full Name intraflagellar transport 52 homolog (Chlamydomonas)
Calculated molecular weight 437 aa, 50 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC039831
Gene symbol IFT52
Gene ID (NCBI) 51098
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IFT52, also named as C20orf9 and NGD5, is a component of IFT complex B which is composed of IFT88, IFT57, TRAF3IP1, IFT52, IFT27, HSPB11 and IFT20. IFT52 binds directly to the IFT81/74/27/25 complex. And IFT52 could mediate the interaction between the two subcomplexes.(PMID:21642430) The MW of IFT52 is about 45-54kd.

Protocols

Product Specific Protocols
WB protocol for IFT52 antibody 17534-1-APDownload protocol
IF protocol for IFT52 antibody 17534-1-APDownload protocol
IP protocol for IFT52 antibody 17534-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Hum Mol Genet

Human IFT52 mutations uncover a novel role for the protein in microtubule dynamics and centrosome cohesion.

Authors - Marie Alice Dupont
humanWB

Biol Open

Robust interaction of IFT70 with IFT52-IFT88 in the IFT-B complex is required for ciliogenesis.

Authors - Ryota Takei
humanWB

PLoS One

Characterization of tetratricopeptide repeat-containing proteins critical for cilia formation and function.

Authors - Yanan Xu
humanIF

Sci Rep

Inhibition of Hedgehog signaling suppresses proliferation and microcyst formation of human Autosomal Dominant Polycystic Kidney Disease cells.

Authors - Luciane M Silva
humanWB

Sci Rep

Destabilization of the IFT-B cilia core complex due to mutations in IFT81 causes a Spectrum of Short-Rib Polydactyly Syndrome.

Authors - Ivan Duran
mouseIF

FASEB J

Genetic interaction of mammalian IFT-A paralogs regulates cilia disassembly, ciliary entry of membrane protein, Hedgehog signaling, and embryogenesis.

Authors - Wei Wang