IGF2BP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

IGF2BP2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

147

Reviews (1)

Cat no : 11601-1-AP

Synonyms

IGF II mRNA binding protein 2, IGF2 mRNA binding protein 2, IGF2BP2, IMP 2, IMP2, p62, VICKZ family member 2, VICKZ2

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Validation Data Gallery

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  WB analysis using 11601-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11601-1-AP (IGF2BP2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 11601-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11601-1-AP (IGF2BP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11601-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11601-1-AP (IGF2BP2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 11601-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11601-1-AP (IGF2BP2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 11601-1-AP

  IP result of anti-IGF2BP2 (IP:11601-1-AP, 3ug; Detection:11601-1-AP 1:500) with HEK-293 cells lysate 1000ug.

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  IHC staining of human pancreas cancer using 11601-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11601-1-AP (IGF2BP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 11601-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11601-1-AP (IGF2BP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11601-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11601-1-AP (IGF2BP2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11601-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11601-1-AP (IGF2BP2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 11601-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 11601-1-AP (IGF2BP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 11601-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 11601-1-AP (IGF2BP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11601-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11601-1-AP (IGF2BP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11601-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11601-1-AP (IGF2BP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, mouse kidney tissue, rat kidney tissue, human kidney tissue, human heart tissue, COLO 320 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human pancreas cancer tissue, human colon cancer tissue, mouse stomach tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 35 publications below
• WB: See 106 publications below
• IHC: See 32 publications below
• IF: See 18 publications below
• IP: See 11 publications below
• CoIP: See 4 publications below
• RIP: See 49 publications below

Product Information

11601-1-AP targets IGF2BP2 in WB, IHC, IF, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IGF2BP2 fusion protein Ag2142
• Full Name: insulin-like growth factor 2 mRNA binding protein 2
• Calculated Molecular Weight: 594 aa, 65 kDa
• Observed Molecular Weight: 55-65 kDa
• GenBank Accession Number: BC021290
• Gene Symbol: IGF2BP2
• Gene ID (NCBI): 10644
• RRID: AB_2122672
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Insulin-like growth factor 2 mRNA-binding protein 2(IGF2BP2), ia a member of a family of mRNA binding protein, which can bind to several mammalian cells' mRNAs. Thus it may involve in the regulation of translation of mRNA. IGF2BP2's polymorphisms is risk factor for developing type 2 diabetes. This antibody raise against part of N-terminus of human IGF2BP2. IGF2BP2 has some isoforms with the MW of 54-66 kDa. Some literature has reported that multiple bands can be detected (PMID: 22427968, 36322753, 23069990).

Publications

Species	Application	Title
human	WB	Cell; An in vitro system to silence mitochondrial gene expression.; Authors - Luis Daniel Cruz-Zaragoza
human	IF	Mol Cancer; m6A methylation reader IGF2BP2 activates endothelial cells to promote angiogenesis and metastasis of lung adenocarcinoma; Authors - Han Fang
mouse	WB	Cell Mol Immunol; Inhibition of METTL3 in macrophages provides protection against intestinal inflammation; Authors - Huilong Yin
human	WB,CoIP,RIP	Cell Res; Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.; Authors - Fei Peng ; KD Validated
mouse	RIP	Nat Cell Biol; N6-methyladenosine regulates maternal RNA maintenance in oocytes and timely RNA decay during mouse maternal-to-zygotic transition.; Authors - You Wu
human	WB,IF	Mol Cell; m6A modification negatively regulates translation by switching mRNA from polysome to P-body via IGF2BP3; Authors - Ting Shan ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Parisa (Verified Customer) (06-13-2022)

good quality and specify.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hek293