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IGFBP3 Polyclonal antibody

IGFBP3 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (2)





Cat no : 10189-2-AP


BP 53, IBP 3, IBP3, IGF binding protein 3, IGFBP 3, IGFBP3

Tested Applications

Positive WB detected inmouse colon tissue, HeLa cells, A2780 cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10189-2-AP targets IGFBP3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IGFBP3 fusion protein Ag0242
Full Name insulin-like growth factor binding protein 3
Calculated molecular weight 32 kDa
Observed molecular weight 32-37 kDa
GenBank accession numberBC000013
Gene symbol IGFBP3
Gene ID (NCBI) 3486
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Insulin-like growth factor binding protein-3 (IGFBP-3) contains a 27 amino acid putative signal sequence followed by a mature protein of 264 amino acids with 18 cysteine residues clustered near the N- and C-terminus. Accordingly, expression of the cloned IGFBP-3 cDNA in mammalian tissue culture cells results in secretion of the protein into the culture medium. IGFBP-3 shares high homology (33% amino acid identity) including conservation of all 18 cysteine residues with a smaller human IGF-binding protein (BP-28) identified in amniotic fluid. IGFBP-3 has one or more glycosylation sites with a protein core of ∼30 kDa. Western blots revealed that the 39-45 kDa IGFBP-3 fragment is a glycoprotein. (PMID: 23403918, PMID: 24926947, PMID: 19887447)


Product Specific Protocols
WB protocol for IGFBP3 antibody 10189-2-APDownload protocol
IHC protocol for IGFBP3 antibody 10189-2-APDownload protocol
IF protocol for IGFBP3 antibody 10189-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Acta Physiol (Oxf)

Podocyte apoptosis in diabetic nephropathy by BASP1 activation of the p53 pathway via WT1.

Authors - Yingying Zhang

Environ Toxicol Pharmacol

Protective potential of epigallocatechin-3-gallate against benign prostatic hyperplasia in metabolic syndrome rats.

Authors - Jinglou Chen

PLoS One

Wound healing with topical BRAF inhibitor therapy in a diabetic model suggests tissue regenerative effects.

Authors - Helena Escuin-Ordinas

J Cancer

Downregulation of LOX promotes castration-resistant prostate cancer progression via IGFBP3.

Authors - Xuanrong Chen

Int J Oncol

Polyphyllin I inhibits invasion and epithelial-mesenchymal transition via CIP2A/PP2A/ERK signaling in prostate cancer.

Authors - Xuewen Liu

Stem Cell Res

Normoxia is not favorable for maintaining stemness of human endothelial progenitor cells.

Authors - Yiqiu Lin