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  • KD/KO Validated

IGFBP3 Polyclonal antibody

IGFBP3 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (2)





Cat no : 10189-2-AP


BP 53, IBP 3, IBP3, IGF binding protein 3, IGFBP 3, IGFBP3

Tested Applications

Positive WB detected inmouse colon tissue, HeLa cells, A2780 cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10189-2-AP targets IGFBP3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IGFBP3 fusion protein Ag0242
Full Name insulin-like growth factor binding protein 3
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32-37 kDa
GenBank Accession NumberBC000013
Gene Symbol IGFBP3
Gene ID (NCBI) 3486
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Insulin-like growth factor binding protein-3 (IGFBP-3) contains a 27 amino acid putative signal sequence followed by a mature protein of 264 amino acids with 18 cysteine residues clustered near the N- and C-terminus. Accordingly, expression of the cloned IGFBP-3 cDNA in mammalian tissue culture cells results in secretion of the protein into the culture medium. IGFBP-3 shares high homology (33% amino acid identity) including conservation of all 18 cysteine residues with a smaller human IGF-binding protein (BP-28) identified in amniotic fluid. IGFBP-3 has one or more glycosylation sites with a protein core of ∼30 kDa. Western blots revealed that the 39-45 kDa IGFBP-3 fragment is a glycoprotein. (PMID: 23403918, PMID: 24926947, PMID: 19887447)


Product Specific Protocols
WB protocol for IGFBP3 antibody 10189-2-APDownload protocol
IHC protocol for IGFBP3 antibody 10189-2-APDownload protocol
IF protocol for IGFBP3 antibody 10189-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Differential effect of cancer-associated fibroblast-derived extracellular vesicles on cisplatin resistance in oral squamous cell carcinoma via miR-876-3p

Authors - Soo Hyun Kang
  • KD Validated

Proc Natl Acad Sci U S A

Disruption of the AMPK-TBC1D1 nexus increases lipogenic gene expression and causes obesity in mice via promoting IGF1 secretion.

Authors - Liang Chen

Acta Physiol (Oxf)

Podocyte apoptosis in diabetic nephropathy by BASP1 activation of the p53 pathway via WT1.

Authors - Yingying Zhang

Cancer Lett

Choline-induced SLC5A7 impairs colorectal cancer growth by stabilizing p53 protein.

Authors - Yuan Yin

PLoS Biol

The glucose-sensing transcription factor MLX balances metabolism and stress to suppress apoptosis and maintain spermatogenesis.

Authors - Patrick A Carroll

J Cell Physiol

Interleukin-33 alleviates diabetic cardiomyopathy through regulation of endoplasmic reticulum stress and autophagy via insulin-like growth factor-binding protein 3.

Authors - Mao-Xiong Wu
  • KD Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Margarita (Verified Customer) (04-19-2023)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: PC3 cells
IGFBP3 Antibody Immunofluorescence validation (1:100 dilution) in PC3 cells (Cat no:10189-2-AP)