IGSF8/CD316 Polyclonal antibody

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  WB analysis of K-562 using 14387-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14387-1-AP (IGSF8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14387-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14387-1-AP (IGSF8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14387-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14387-1-AP (IGSF8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14387-1-AP

  IP result of anti-IGSF8/CD316 (IP:14387-1-AP, 3ug; Detection:14387-1-AP 1:500) with mouse brain tissue lysate 5000ug.

IGSF8/CD316 Polyclonal Antibody for WB, IP, ELISA

Cat No. 14387-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

CD316, CD81 partner 3, CD81P3, EWI 2, EWI2, IGSF8, KCT 4, KCT4, LIR D1, PGRL

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Tested Applications

• Positive WB detected in: K-562 cells, HEK-293 cells, mouse brain tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

14387-1-AP targets IGSF8/CD316 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IGSF8/CD316 fusion protein Ag5846
• Full Name: immunoglobulin superfamily, member 8
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC053881
• Gene Symbol: IGSF8
• Gene ID (NCBI): 93185
• RRID: AB_2121881
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q969P0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGSF8 is also known as CD316, PGRL (PG regulatory-like protein), KAI/CD82-associated surface molecule, and CD81-binding partner 3 (CD81P3). IGSF8 contains four immunoglobulin domains, a transmembrane region, and a short cytoplasmic tail that does not bear any signature motif for signal transduction. IGSF8 which is abundantly expressed in olfactory sensory neuron (OSN) axons and their developing synapses. (PMID: 17785435, PMID: 22687584)

Publications

Species	Application	Title
rat	WB	Mol Ther Nucleic Acids; Exosomal miR-365a-5p derived from HUC-MSCs regulates osteogenesis in GIONFH through the Hippo signaling pathway.; Authors - Ming-Jie Kuang
rat	WB	Biochem Biophys Res Commun; Human umbilical cord mesenchymal stem cell-derived exosomes act via the miR-1263/Mob1/Hippo signaling pathway to prevent apoptosis in disuse osteoporosis.; Authors - Bao-Cheng Yang