IGSF8/CD316 Polyclonal antibody

IGSF8/CD316 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 14387-1-AP

Synonyms

CD316, CD81 partner 3, CD81P3, EWI 2, EWI2, IGSF8, KCT 4, KCT4, LIR D1, PGRL



Tested Applications

Positive WB detected inK-562 cells, HEK-293 cells, mouse brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

14387-1-AP targets IGSF8/CD316 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IGSF8/CD316 fusion protein Ag5846
Full Name immunoglobulin superfamily, member 8
Calculated molecular weight 65 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC053881
Gene symbol IGSF8
Gene ID (NCBI) 93185
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IGSF8 is also known as CD316, PGRL (PG regulatory-like protein), KAI/CD82-associated surface molecule, and CD81-binding partner 3 (CD81P3). IGSF8 contains four immunoglobulin domains, a transmembrane region, and a short cytoplasmic tail that does not bear any signature motif for signal transduction. IGSF8 which is abundantly expressed in olfactory sensory neuron (OSN) axons and their developing synapses. (PMID: 17785435, PMID: 22687584)

Protocols

Product Specific Protocols
WB protocol for IGSF8/CD316 antibody 14387-1-APDownload protocol
IP protocol for IGSF8/CD316 antibody 14387-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cell Death Dis

KLF4-mediated upregulation of CD9 and CD81 suppresses hepatocellular carcinoma development via JNK signaling.

Authors - Yandong Li
  • KD Validated
ratWB

Biochem Biophys Res Commun

Human umbilical cord mesenchymal stem cell-derived exosomes act via the miR-1263/Mob1/Hippo signaling pathway to prevent apoptosis in disuse osteoporosis.

Authors - Bao-Cheng Yang
mouseWB

Int J Biol Sci

Exosomes derived from Wharton's jelly of human umbilical cord mesenchymal stem cells reduce osteocyte apoptosis in glucocorticoid-induced osteonecrosis of the femoral head in rats via the miR-21-PTEN-AKT signalling pathway.

Authors - Ming-Jie Kuang