Tested Applications
| Positive WB detected in | Jurkat cells, mouse brain tissue |
| Positive IHC detected in | human kidney tissue, human lung tissue, mouse brain tissue, mouse lung tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse embryo tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 31 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| IP | See 3 publications below |
| CoIP | See 2 publications below |
Product Information
18474-1-AP targets IKBKG in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IKBKG fusion protein Ag13358 Predict reactive species |
| Full Name | inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC012114 |
| Gene Symbol | IKBKG |
| Gene ID (NCBI) | 8517 |
| RRID | AB_10646443 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6K9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IKBKG, also named as FIP3, NEMO, IKKAP1 and IKKG, is specifically phosphorylate serine or threonine residues that are followed by a proline residue. IKBKG is regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. IKBKG is a predominant 48-kD protein and an N-terminally truncated protein of 45 kDa produced in smaller amounts and translated from methionine-38.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IKBKG antibody 18474-1-AP | Download protocol |
| IHC protocol for IKBKG antibody 18474-1-AP | Download protocol |
| IF protocol for IKBKG antibody 18474-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Biol Caspase inhibition prolongs inflammation by promoting a signaling complex with activated RIPK1. | ||
Cell Death Differ IKK-β mediates hydrogen peroxide induced cell death through p85 S6K1.
| ||
Mol Cell Proteomics Quantification of Dynamic Protein Interactions and Phosphorylation in LPS Signaling Pathway by SWATH-MS | ||
Front Immunol PCBP1 interacts with the HTLV-1 Tax oncoprotein to potentiate NF-κB activation | ||
FASEB J 3D human bone marrow stromal and endothelial cell spheres promote bone healing in an osteogenic niche. |
