Tested Applications
| Positive WB detected in | Jurkat cells, mouse brain tissue |
| Positive IHC detected in | human placenta tissue, mouse brain tissue, human lung tissue, mouse liver tissue, mouse lung tissue, rat liver tissue, rat lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18474-1-AP targets IKBKG in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13358 Product name: Recombinant human IKBKG protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 120-419 aa of BC012114 Sequence: QALREVEHLKRCQQQMAEDKASVKAQVTSLLGELQESQSRLEAATKECQALEGRARAASEQARQLESEREALQQQHSVQVDQLRMQGQSVEAALRMERQAASEEKRKLAQLQVAYHQLFQEYDNHIKSSVVGSERKRGMQLEDLKQQLQQAEEALVAKQEVIDKLKEEAEQHKIVMETVPVLKAQADIYKADFQAERQAREKLAEKKELLQEQLEQLQREYSKLKASCQESARIEDMRKRHVEVSQAPLPPAPAYLSSPLALPSQRRSPPEEPPDFCCPKCQYQAPDMDTLQIHVMECIE Predict reactive species |
| Full Name | inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC012114 |
| Gene Symbol | IKBKG |
| Gene ID (NCBI) | 8517 |
| RRID | AB_10646443 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6K9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IKBKG, also named as FIP3, NEMO, IKKAP1 and IKKG, is specifically phosphorylate serine or threonine residues that are followed by a proline residue. IKBKG is regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. IKBKG is a predominant 48-kD protein and an N-terminally truncated protein of 45 kDa produced in smaller amounts and translated from methionine-38.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IKBKG antibody 18474-1-AP | Download protocol |
| IHC protocol for IKBKG antibody 18474-1-AP | Download protocol |
| WB protocol for IKBKG antibody 18474-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Antibody: IKBKG Polyclonal antibody (Cat# 18474-1-AP). Total citations: 35. Journals include Nature Communications, Cell Death & Differentiation, Theranostics, Molecular & Cellular Proteomics, Journal of Cell Biology, FASEB Journal, Journal of Virology, Brain, Behavior, and Immunity, Frontiers in Immunology, Phytomedicine, and others. Research fields span NF-κB signaling, inflammation, virology, oncology, neuroinflammation, apoptosis, atherosclerosis, and liver disease.
| Species | Application | Title |
|---|---|---|
Nat Commun Loss-of-function mutations in Keratin 32 gene disrupt skin immune homeostasis in pityriasis rubra pilaris | ||
Theranostics MiR-125a-5p in extracellular vesicles of neural stem cells acts as a crosstalk signal modulating neuroinflammatory microenvironment to alleviate cerebral ischemia-reperfusion injury. | ||
Cell Death Differ IKK-β mediates hydrogen peroxide induced cell death through p85 S6K1.
| ||
Phytomedicine Arteannuin B, a sesquiterpene lactone from Artemisia annua, attenuates inflammatory response by inhibiting the ubiquitin-conjugating enzyme UBE2D3-mediated NF-κB activation | ||
Brain Behav Immun Cannabidiol alleviates neuroinflammation and attenuates neuropathic pain via targeting FKBP5 |
Reviews
What customers say
Both reviewers gave 5-star ratings. One user reported excellent performance in western blot and immunofluorescence, validated using IKBKG knockout cells with U2OS and HeLa cell lines at a 1:1000 dilution. The other user successfully applied the antibody for western blot and immunohistochemistry on Caco2 cells and animal colon tissue, also at 1:1000 dilution. Overall, users find this antibody reliable across multiple applications including WB, IF, and IHC, with consistent results in both human cell lines and tissue samples.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Emmanuel (Verified Customer) (11-28-2025) | Great antibody for western blot and immunofluorescence. Validated using IKBKG KO cells
|
P (Verified Customer) (07-15-2025) | Used for caco2 cells and animal colon tissue
|
