- Featured Product
- KD/KO Validated
IKBKG Polyclonal antibody
IKBKG Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IHC, IF, ELISA
Cat no : 18474-1-AP
|Positive WB detected in||Jurkat cells, mouse brain tissue|
|Positive IHC detected in||human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, mouse embryo tissue|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 4 publications below|
|WB||See 26 publications below|
|IF||See 1 publications below|
|IP||See 2 publications below|
The immunogen of 18474-1-AP is IKBKG Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IKBKG fusion protein Ag13358|
|Full Name||inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma|
|Calculated molecular weight||48 kDa|
|Observed molecular weight||48 kDa|
|GenBank accession number||BC012114|
|Gene ID (NCBI)||8517|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
IKBKG, also named as FIP3, NEMO, IKKAP1 and IKKG, is specifically phosphorylate serine or threonine residues that are followed by a proline residue. IKBKG is regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. IKBKG is a predominant 48-kD protein and an N-terminally truncated protein of 45 kDa produced in smaller amounts and translated from methionine-38.
|Product Specific Protocols|
|WB protocol for IKBKG antibody 18474-1-AP||Download protocol|
|IHC protocol for IKBKG antibody 18474-1-AP||Download protocol|
|IF protocol for IKBKG antibody 18474-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Cell Biol
Caspase inhibition prolongs inflammation by promoting a signaling complex with activated RIPK1.
Cell Death Differ
IKK-β mediates hydrogen peroxide induced cell death through p85 S6K1.
RIP1 modulates death receptor mediated apoptosis and autophagy in macrophages.
3D human bone marrow stromal and endothelial cell spheres promote bone healing in an osteogenic niche.
Cell Mol Immunol
Corilagin Ameliorates Atherosclerosis in Peripheral Artery Disease via the Toll-Like Receptor-4 Signaling Pathway in vitro and in vivo.