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  • KD/KO Validated

IKBKG Polyclonal antibody

IKBKG Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 18474-1-AP


AMCBX1, FIP 3, FIP3, Fip3p, I kappa B kinase subunit gamma, IkB kinase subunit gamma, IKBKG, IKK gamma, IKKAP1, IKKG, IP, IP1, IP2, IPD2, NEMO, NF kappa B essential modifier, NF kappa B essential modulator

Tested Applications

Positive WB detected inJurkat cells, mouse brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, mouse embryo tissue

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 18474-1-AP is IKBKG Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IKBKG fusion protein Ag13358
Full Name inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC012114
Gene Symbol IKBKG
Gene ID (NCBI) 8517
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IKBKG, also named as FIP3, NEMO, IKKAP1 and IKKG, is specifically phosphorylate serine or threonine residues that are followed by a proline residue. IKBKG is regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. IKBKG is a predominant 48-kD protein and an N-terminally truncated protein of 45 kDa produced in smaller amounts and translated from methionine-38.


Product Specific Protocols
WB protocol for IKBKG antibody 18474-1-APDownload protocol
IHC protocol for IKBKG antibody 18474-1-APDownload protocol
IF protocol for IKBKG antibody 18474-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Cell Biol

Caspase inhibition prolongs inflammation by promoting a signaling complex with activated RIPK1.

Authors - Xinyue Huang

Cell Death Differ

IKK-β mediates hydrogen peroxide induced cell death through p85 S6K1.

Authors - Jia C-H CH
  • KD Validated

Mol Cell Proteomics

Quantification of Dynamic Protein Interactions and Phosphorylation in LPS Signaling Pathway by SWATH-MS

Authors - Xiurong Wu

Mol Oncol

RIP1 modulates death receptor mediated apoptosis and autophagy in macrophages.

Authors - Zhenyu Yao


3D human bone marrow stromal and endothelial cell spheres promote bone healing in an osteogenic niche.

Authors - Stefanie Inglis

Cell Mol Immunol

PMID: 28392573

Authors - Yushun Wan