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IKBKG Polyclonal antibody

IKBKG Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 18474-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

AMCBX1, FIP3, FIP-3, Fip3p, I-kappa-B kinase subunit gamma

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inJurkat cells, mouse brain tissue
Positive IHC detected inhuman placenta tissue, mouse brain tissue, human lung tissue, mouse liver tissue, mouse lung tissue, rat liver tissue, rat lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18474-1-AP targets IKBKG in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IKBKG fusion protein Ag13358 Predict reactive species
Full Name inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC012114
Gene Symbol IKBKG
Gene ID (NCBI) 8517
RRIDAB_10646443
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6K9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IKBKG, also named as FIP3, NEMO, IKKAP1 and IKKG, is specifically phosphorylate serine or threonine residues that are followed by a proline residue. IKBKG is regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. IKBKG is a predominant 48-kD protein and an N-terminally truncated protein of 45 kDa produced in smaller amounts and translated from methionine-38.

Protocols

Product Specific Protocols
WB protocol for IKBKG antibody 18474-1-APDownload protocol
IHC protocol for IKBKG antibody 18474-1-APDownload protocol
IF protocol for IKBKG antibody 18474-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Cell Biol

Caspase inhibition prolongs inflammation by promoting a signaling complex with activated RIPK1.

Authors - Xinyue Huang
humanWB

Cell Death Differ

IKK-β mediates hydrogen peroxide induced cell death through p85 S6K1.

Authors - Jia C-H CH
  • KD Validated
humanWB

Mol Cell Proteomics

Quantification of Dynamic Protein Interactions and Phosphorylation in LPS Signaling Pathway by SWATH-MS

Authors - Xiurong Wu
humanWB

Front Immunol

PCBP1 interacts with the HTLV-1 Tax oncoprotein to potentiate NF-κB activation

Authors - Rui Su
humanWB

Mol Oncol

RIP1 modulates death receptor mediated apoptosis and autophagy in macrophages.

Authors - Zhenyu Yao
humanWB,IF

FASEB J

3D human bone marrow stromal and endothelial cell spheres promote bone healing in an osteogenic niche.

Authors - Stefanie Inglis
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