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IKBKG Antibody 12 Publications

Rabbit Polyclonal| Catalog number: 18474-1-AP

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Con: 29 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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IKBKG Antibody IF mouse embryo tissue 18474-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed mouse embryo tissue using 18474-1-AP (IKBKG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

IKBKG Antibody WB K-562 cells 18474-1-AP

K-562 cells were subjected to SDS PAGE followed by western blot with 18474-1-AP(IKBKG antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours

IKBKG Antibody IHC human kidney tissue 18474-1-AP

Immunohistochemical analysis of paraffin-embedded human kidney using 18474-1-AP(IKBKG antibody) at dilution of 1:50 (under 10x lens)

IKBKG Antibody IHC human kidney tissue 18474-1-AP

Immunohistochemical analysis of paraffin-embedded human kidney using 18474-1-AP(IKBKG antibody) at dilution of 1:50 (under 40x lens)

IKBKG Antibody IF HeLa cells 18474-1-AP

Immunofluorescent analysis of HeLa cells using 18474-1-AP(IKBKG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
K-562 cells, HEK-293 cells, mouse brain tissue
Positive IHC detected in:
human kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells, mouse embryo tissue
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:20-1:200
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
IKBKG fusion protein Ag13358
Full name:
inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma
Calculated molecular weight:
48 kDa
Observed molecular weight:
48 kDa
GenBank accession number:
BC012114
Gene ID (NCBI):
8517
Gene symbol
IKBKG
Synonyms
AMCBX1, FIP 3, FIP3, Fip3p, I kappa B kinase subunit gamma, IkB kinase subunit gamma, IKBKG, IKK gamma, IKKAP1, IKKG, IP, IP1, IP2, IPD2, NEMO, NF kappa B essential modifier, NF kappa B essential modulator

Background

IKBKG, also named as FIP3, NEMO, IKKAP1 and IKKG, is specifically phosphorylate serine or threonine residues that are followed by a proline residue. IKBKG is regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. IKBKG is a predominant 48-kD protein and an N-terminally truncated protein of 45 kDa produced in smaller amounts and translated from methionine-38.


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