IL-10 Polyclonal antibody

IL-10 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20850-1-AP

Synonyms

CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF



Tested Applications

Positive WB detected inRecombinant protein, ag14644

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20850-1-AP is IL-10 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, monkey, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-10 fusion protein Ag14644
Full Name interleukin 10
Calculated molecular weight 21 kDa
GenBank accession numberBC104252
Gene symbol IL10
Gene ID (NCBI) 3586
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The human IL10 gene is 4.7 kb in size and is located on the long arm of chromosome 1. It contains five exons. Biologically functional IL-10 exists in the form of a 36 kDa homodimer composed of two non-covalently bonded mono-mers each with 160 amino acids length. IL-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

Protocols

Product Specific Protocols
WB protocol for IL-10 antibody 20850-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Commun

B cells sustain inflammation and predict response to immune checkpoint blockade in human melanoma.

Authors - Johannes Griss
mouseIHC

Microbiome

Depletion of acetate-producing bacteria from the gut microbiota facilitates cognitive impairment through the gut-brain neural mechanism in diabetic mice.

Authors - Hong Zheng
mouseWB

J Control Release

Peritoneal M2 macrophage-derived extracellular vesicles as natural multitarget nanotherapeutics to attenuate cytokine storms after severe infections.

Authors - Yizhuo Wang
humanIHC

J Control Release

In situ hydrogel capturing nitric oxide microbubbles accelerates the healing of diabetic foot

Authors - Yingzheng Zhao
mouseWB

Carbohydr Polym

Chemical structure and anti-inflammatory activity of a branched polysaccharide isolated from Phellinus baumii.

Authors - Yuqing Sun
ratIF

Brain Behav Immun

Microglial IL-10 and β-endorphin expression mediates gabapentinoids antineuropathic pain.

Authors - Khalil Ali Ahmad