IL-10 Polyclonal antibody

IL-10 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG


human and More (5)





Cat no : 20850-1-AP


CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF

Tested Applications

Positive WB detected inRecombinant protein, ag14644

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20850-1-AP is IL-10 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, monkey, pig, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-10 fusion protein Ag14644
Full Name interleukin 10
Calculated Molecular Weight 21 kDa
GenBank Accession NumberBC104252
Gene Symbol IL10
Gene ID (NCBI) 3586
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The human IL10 gene is 4.7 kb in size and is located on the long arm of chromosome 1. It contains five exons. Biologically functional IL-10 exists in the form of a 36 kDa homodimer composed of two non-covalently bonded mono-mers each with 160 amino acids length. IL-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.


Product Specific Protocols
WB protocol for IL-10 antibody 20850-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Commun

B cells sustain inflammation and predict response to immune checkpoint blockade in human melanoma.

Authors - Johannes Griss


Depletion of acetate-producing bacteria from the gut microbiota facilitates cognitive impairment through the gut-brain neural mechanism in diabetic mice.

Authors - Hong Zheng

Acta Biomater

A KPV-binding double-network hydrogel restores gut mucosal barrier in an inflamed colon.

Authors - Yingzheng Zhao

Acta Biomater

Bio-inspired Glycosylated Nano-hydroxyapatites Enhance Endogenous Bone Regeneration by Modulating Macrophage M2 Polarization

Authors - Dan Qiao

Int J Biol Macromol

In situ polyphenol-adhesive hydrogel enhanced the noncarcinogenic repairing of KGF on the gut epithelial barrier on TNBS-induced colitis rats

Authors - Gaolong Lin

J Control Release

In situ hydrogel capturing nitric oxide microbubbles accelerates the healing of diabetic foot

Authors - Yingzheng Zhao