IL-12/IL-23 p40 Polyclonal antibody

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  WB analysis using 30537-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30537-1-AP (IL-12/IL-23 p40 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

IL-12/IL-23 p40 Polyclonal Antibody for WB, ELISA

Cat No. 30537-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

Il12b, Cytotoxic lymphocyte maturation factor 40 kDa subunit, Il 12b, Il 12p40, IL-12 p40

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse heart tissue, mouse kidney tissue, rat brain tissue, rat heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30537-1-AP targets IL-12/IL-23 p40 in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 12b
• Calculated Molecular Weight: 38kd
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: NM_001303244
• Gene Symbol: Il12b
• Gene ID (NCBI): 16160
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P43432
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin (IL)-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).