IL-1 Alpha Polyclonal antibody

IL-1 Alpha Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)





Cat no : 16765-1-AP


Hematopoietin 1, IL 1 alpha, IL 1A, IL1, IL-1, IL1 ALPHA, IL-1 alpha, IL1A, IL1F1, IL-1α, Interleukin 1 alpha, interleukin 1, alpha

Tested Applications

Positive WB detected inPMA, LPS and Brefeldin A treated THP-1 cells, human placenta tissue, mouse liver tissue, mouse lung tissue
Positive IHC detected inhuman appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inRAW 264.7 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16765-1-AP targets IL-1 Alpha in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-1 Alpha fusion protein Ag10339
Full Name interleukin 1, alpha
Calculated Molecular Weight 271 aa, 31 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC013142
Gene Symbol IL1A
Gene ID (NCBI) 3552
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-1α (IL-1 Alpha) is a protein that plays a crucial role in the regulation of a number of key cellular processes. IL-1 Alpha is a cytokine and a potent mediator of body's response to inflammation, microbial invasion, tissue injury and immunological response. In addition, recent studies suggest that IL-1 Alpha also has a role in wound healing, rheumatoid arthritis, Alzheimer's disease and tumor growth. IL-1 Alpha functions as an alarmin during the process of sterile inflammation. It serves as a signal from dying cells to potentiate the inflammatory response, increasing the expression of other secreted factors like IL-6 and IL-8. IL-1 Alpha was found to affect the cell cycle of osteosarcoma cell to reduce cell growth. IL-1 Alpha is initially translated as approximately 30-kD polypeptides that are processed to approximately 17.5-kD polypeptides prior to, or during, release from macrophages.


Product Specific Protocols
WB protocol for IL-1 Alpha antibody 16765-1-APDownload protocol
IHC protocol for IL-1 Alpha antibody 16765-1-APDownload protocol
IF protocol for IL-1 Alpha antibody 16765-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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