IL-1 Alpha Polyclonal antibody

IL-1 Alpha Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 16765-1-AP

Synonyms

Hematopoietin 1, IL 1 alpha, IL 1A, IL1, IL1 ALPHA, IL-1 alpha, IL1A, IL1F1, IL-1α, Interleukin 1 alpha, interleukin 1, alpha



Tested Applications

Positive WB detected inmouse liver tissue, mouse lung tissue, Jurkat cells, human placenta tissue
Positive IF detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 16765-1-AP is IL-1 Alpha Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-1 Alpha fusion protein Ag10339
Full Name interleukin 1, alpha
Calculated molecular weight 271 aa, 31 kDa
Observed molecular weight 30 kDa, 17 kDa
GenBank accession numberBC013142
Gene symbol IL1A
Gene ID (NCBI) 3552
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-1α (IL-1α) is a protein that plays a crucial role in the regulation of a number of key cellular processes. IL-1αis a cytokine and a potent mediator of body's response to inflammation, microbial invasion, tissue injury and immunological response. In addition, recent studies suggest that IL-1αalso has a role in wound healing, rheumatoid arthritis, Alzheimer's disease and tumor growth. IL-1αfunctions as an alarmin during the process of sterile inflammation. It serves as a signal from dying cells to potentiate the inflammatory response, increasing the expression of other secreted factors like IL-6 and IL-8. IL-1αwas found to affect the cell cycle of osteosarcoma cell to reduce cell growth. IL1-alpha is initially translated as approximately 30-kD polypeptides that are processed to approximately 17.5-kD polypeptides prior to, or during, release from macrophages.

Protocols

Product Specific Protocols
WB protocol for IL-1 Alpha antibody 16765-1-APDownload protocol
IF protocol for IL-1 Alpha antibody 16765-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Exp Ther Med

TLR4 gene in the regulation of periodontitis and its molecular mechanism.

Authors - Weijuan Qi
mouseIHC

Naunyn Schmiedebergs Arch Pharmacol

Crocin attenuates isoprenaline-induced myocardial fibrosis by targeting TLR4/NF-κB signaling: connecting oxidative stress, inflammation, and apoptosis.

Authors - Weiyue Jin
ratIHC

Mol Med Rep

Ameliorative effects and mechanism of crocetin in arsenic trioxide‑induced cardiotoxicity in rats.

Authors - Zhifeng Zhao
mouseIHC

J Tissue Viability

Non-invasive detection of local tissue responses to predict pressure ulcer development in mouse models.

Authors - Nao Kimura
humanWB

Int Immunopharmacol

PMID: 31220697

Authors - Jie Chen
mouseWB,IHC

Front Pharmacol

Effects of Ketamine on Levels of Inflammatory Cytokines IL-6, IL-1β, and TNF-α in the Hippocampus of Mice Following Acute or Chronic Administration.

Authors - Yanning Li