IL-1RL1/ST2 Polyclonal antibody

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  WB analysis using 11920-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11920-1-AP (ST2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 11920-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11920-1-AP (ST2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11920-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11920-1-AP (ST2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 11920-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11920-1-AP (ST2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 11920-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11920-1-AP (ST2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 11920-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11920-1-AP (ST2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 11920-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11920-1-AP (ST2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IL-1RL1/ST2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11920-1-AP

Publications(38)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

IL1RL1, IL-33R, IL-1RL1, FIT 1, EC:3.2.2.6

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Tested Applications

• Positive WB detected in: human placenta tissue, mouse lung tissue, rat lung tissue
• Positive IHC detected in: mouse heart tissue, human lung cancer tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 21 publications below
• IHC: See 16 publications below
• IF: See 14 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

11920-1-AP targets IL-1RL1/ST2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-1RL1/ST2 fusion protein Ag2555
• Full Name: interleukin 1 receptor-like 1
• Calculated Molecular Weight: 63 kDa, 37 kDa, 30 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC030975
• Gene Symbol: IL1RL1
• Gene ID (NCBI): 9173
• RRID: AB_906359
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q01638
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ST2, also known as IL1RL1, is a member of the IL-1 superfamily and serves as the receptor for IL-33. It plays a major role in immune and inflammatory responses. ST2 is expressed by various types of immune cells including Th2 cells, mast cells, type 2 innate lymphoid cells, eosinophils, basophils, NK cells, and non-immune cells, including cardiomyocytes. Two forms of ST2 exist: a membrane-bound form and a soluble form (sST2). The membrane-bound form activates the MyD88/NF-κB signaling pathway to enhance mast cell, Th2, regulatory T cell (Treg), and innate lymphoid cell type 2 functions. sST2 acts as a decoy receptor. (PMID: 27055914; 23999434; 28484466)

Publications

Species	Application	Title
mouse	IF,WB	Adv Healthc Mater; Ultrasmall Nanoparticles Regulate Immune Microenvironment by Activating IL-33/ST2 to Alleviate Renal Ischemia-Reperfusion Injury; Authors - Liyao Xu
human	IHC	Proc Natl Acad Sci U S A; IL-33 activates tumor stroma to promote intestinal polyposis.; Authors - Rebecca L Maywald
human,mouse	WB,IP,CoIP	Cell Commun Signal; IL-33/ST2 antagonizes STING signal transduction via autophagy in response to acetaminophen-mediated toxicological immunity; Authors - Zengbin Wang
human	IHC,IF	J Invest Dermatol; Increased Type 2 Innate Lymphoid Cells in Patients with Drug Reaction with Eosinophilia and Systemic Symptoms Syndrome.; Authors - Yi-Giien Tsai
human	WB,IF	Oncogene; The reciprocal interaction between tumor cells and activated fibroblasts mediated by TNF-α/IL-33/ST2L signaling promotes gastric cancer metastasis.; Authors - Quan Zhou
human,mouse	WB,IHC	Am J Pathol; Cross Talk between ST2 and Transforming Growth Factor-β Receptor Signaling Promotes Renal Fibrosis; Authors - Xingxing Zhu