ST2 Polyclonal antibody

ST2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11920-1-AP

Synonyms

DER4, FIT 1, IL1RL1, IL-1RL1, IL-33R, interleukin 1 receptor like 1, Protein ST2, ST2, ST2L, ST2V, T1



Tested Applications

Positive WB detected inhuman placenta tissue, mouse lung tissue, rat lung tissue
Positive IHC detected inhuman lung cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11920-1-AP targets ST2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ST2 fusion protein Ag2555
Full Name interleukin 1 receptor-like 1
Calculated molecular weight 63 kDa, 37 kDa, 30 kDa
Observed molecular weight50 kDa
GenBank accession numberBC030975
Gene symbol IL1RL1
Gene ID (NCBI) 9173
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ST2 antibody 11920-1-APDownload protocol
IHC protocol for ST2 antibody 11920-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Proc Natl Acad Sci U S A

IL-33 activates tumor stroma to promote intestinal polyposis.

Authors - Rebecca L Maywald
humanIHC,IF

J Invest Dermatol

Increased Type 2 Innate Lymphoid Cells in Patients with Drug Reaction with Eosinophilia and Systemic Symptoms Syndrome.

Authors - Yi-Giien Tsai
humanWB,IF

Oncogene

The reciprocal interaction between tumor cells and activated fibroblasts mediated by TNF-α/IL-33/ST2L signaling promotes gastric cancer metastasis.

Authors - Quan Zhou
rat,mouseWB,IHC,IF

J Neuroinflammation

Red nucleus IL-33 facilitates the early development of mononeuropathic pain in male rats by inducing TNF-α through activating ERK, p38 MAPK, and JAK2/STAT3.

Authors - Hao-Nan Li
ratWB

J Neuroinflammation

IL-33/ST2 signaling contributes to radicular pain by modulating MAPK and NF-κB activation and inflammatory mediator expression in the spinal cord in rat models of noncompressive lumber disk herniation.

Authors - Si-Jian Huang
mouseIHC

Sci Rep

The miRNA199a/SIRT1/P300/Yy1/sST2 signaling axis regulates adverse cardiac remodeling following MI.

Authors - Maria Carmen Asensio-Lopez