IL-6 Polyclonal antibody

• 

  WB analysis using 21865-1-AP

  Untreated HUVEC cells, HUVEC cells (treated with 0.5 µg/ml LPS for 24 hours, then add 300 ng/ml protein transport inhibitor last 20 hours) were subjected to SDS PAGE followed by western blot with 21865-1-AP (IL-6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HUVEC using 21865-1-AP

  IP result of anti-IL-6 (IP:21865-1-AP, 4ug; Detection:21865-1-AP 1:1000) with LPS and Protein transport inhibitor treated HUVEC cells lysate 800 ug.

• 

  IF Staining of HUVEC using 21865-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed LPS and Brefeldin A treated HUVEC cells using IL-6 antibody (21865-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

IL-6 antibodies are used for both research and therapeutic purposes to detect, quantify, and block the activity of interleukin-6 (IL-6), a cytokine central to immune regulation, inflammation, hematopoiesis, and cancer biology. Proteintech has an extensive range of IL-6 antibodies validated for WB, IHC, IF/ICC, IP, Neutralization, ELISA and more.

Cat No. 21865-1-AP

Publications(729)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (5)

Applications

WB, IHC, IP, Neutralization, ELISA

Synonyms

IL6, Interleukin 6, Interferon beta-2, IL 6, IFN-beta-2

---

Tested Applications

• Positive WB detected in: LPS and Protein transport inhibitor treated HUVEC cells
• Positive IP detected in: LPS and Protein transport inhibitor treated HUVEC cells
• Positive IF/ICC detected in: LPS and Brefeldin A treated HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 109 publications below
• IHC: See 185 publications below
• IF: See 38 publications below
• ELISA: See 5 publications below

Product Information

21865-1-AP targets IL-6 in WB, IHC, IP, Neutralization, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig, rabbit, canine, bovine, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-6 fusion protein Ag10565
• Full Name: interleukin 6 (interferon, beta 2)
• Calculated Molecular Weight: 212 aa, 24 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC015511
• Gene Symbol: IL6
• Gene ID (NCBI): 3569
• ENSEMBL Gene ID: ENSG00000136244
• RRID: AB_11142677
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P05231
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Nat Biotechnol; Magnify is a universal molecular anchoring strategy for expansion microscopy; Authors - Aleksandra Klimas
		J Hematol Oncol; FFAR2 expressing myeloid-derived suppressor cells drive cancer immunoevasion; Authors - Zeda Zhao
	IF	Cancer Cell; Cathepsin C promotes breast cancer lung metastasis by modulating neutrophil infiltration and neutrophil extracellular trap formation.; Authors - Yansen Xiao
	IHC	Bioact Mater; A bioactive composite hydrogel dressing that promotes healing of both acute and chronic diabetic skin wounds; Authors - Shunlai Shang
		Bioact Mater; Genetically engineered M2-like macrophage-derived exosomes for P. gingivalis-suppressed cementum regeneration: From mechanism to therapy; Authors - Xin Huang
		ACS Cent Sci; Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury; Authors - Juan Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Udesh (Verified Customer) (04-19-2023)

Lowest most band corresponds to IL-6

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U2OS

IL-6 Antibody Western Blot validation (1:1000 dilution) in U2OS (Cat no:21865-1-AP)

FH

Elizabeth (Verified Customer) (12-03-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse