IL-6 Polyclonal antibody

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  WB analysis using 21865-1-AP

  Untreated HUVEC cells, HUVEC cells (treated with 0.5 µg/ml LPS for 24 hours, then add 300 ng/ml protein transport inhibitor last 20 hours) were subjected to SDS PAGE followed by western blot with 21865-1-AP (IL-6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HUVEC using 21865-1-AP

  IP result of anti-IL-6 (IP:21865-1-AP, 4ug; Detection:21865-1-AP 1:1000) with LPS and Protein transport inhibitor treated HUVEC cells lysate 800 ug.

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  IF Staining of HUVEC using 21865-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed LPS and Brefeldin A treated HUVEC cells using IL-6 antibody (21865-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

IL-6 antibodies are used for both research and therapeutic purposes to detect, quantify, and block the activity of interleukin-6 (IL-6), a cytokine central to immune regulation, inflammation, hematopoiesis, and cancer biology. Proteintech has an extensive range of IL-6 antibodies validated for WB, IHC, IF/ICC, IP, Neutralization, ELISA and more.

Cat No. 21865-1-AP

Publications(719)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (5)

Applications

WB, IHC, IP, Neutralization, ELISA

Synonyms

IL6, Interleukin 6, Interferon beta-2, IL 6, IFN-beta-2

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Tested Applications

• Positive WB detected in: LPS and Protein transport inhibitor treated HUVEC cells
• Positive IP detected in: LPS and Protein transport inhibitor treated HUVEC cells
• Positive IF/ICC detected in: LPS and Brefeldin A treated HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 102 publications below
• IHC: See 184 publications below
• IF: See 38 publications below
• ELISA: See 5 publications below

Product Information

21865-1-AP targets IL-6 in WB, IHC, IP, Neutralization, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig, rabbit, canine, bovine, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-6 fusion protein Ag10565
• Full Name: interleukin 6 (interferon, beta 2)
• Calculated Molecular Weight: 212 aa, 24 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC015511
• Gene Symbol: IL6
• Gene ID (NCBI): 3569
• ENSEMBL Gene ID: ENSG00000136244
• RRID: AB_11142677
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P05231
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

Publications

Species	Application	Title
human	IF	Nat Biotechnol; Magnify is a universal molecular anchoring strategy for expansion microscopy; Authors - Aleksandra Klimas
		J Hematol Oncol; FFAR2 expressing myeloid-derived suppressor cells drive cancer immunoevasion; Authors - Zeda Zhao
	IF	Cancer Cell; Cathepsin C promotes breast cancer lung metastasis by modulating neutrophil infiltration and neutrophil extracellular trap formation.; Authors - Yansen Xiao
		Bioact Mater; Genetically engineered M2-like macrophage-derived exosomes for P. gingivalis-suppressed cementum regeneration: From mechanism to therapy; Authors - Xin Huang
	IHC	Bioact Mater; A bioactive composite hydrogel dressing that promotes healing of both acute and chronic diabetic skin wounds; Authors - Shunlai Shang
		ACS Cent Sci; Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury; Authors - Juan Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Udesh (Verified Customer) (04-19-2023)

Lowest most band corresponds to IL-6

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U2OS

IL-6 Antibody Western Blot validation (1:1000 dilution) in U2OS (Cat no:21865-1-AP)

FH

Elizabeth (Verified Customer) (12-03-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse