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  • KD/KO Validated

IL-6 Antibody

IL-6 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 21865-1-AP

Synonyms

B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HGF, HSF, Hybridoma growth factor, IFN beta 2, IFNB2, IL 6, IL6, IL-6, Interferon beta 2, Interleukin 6



Tested Applications

Positive IHC detected inhuman tonsillitis tissue, human spleen tissue, human hepatocirrhosis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 21865-1-AP immunogen is Fusion Protein IL-6 protein expressed in E. coli.

Reactivity human
Cited Species dog, goat, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-6 fusion protein Ag10565
Full Name interleukin 6 (interferon, beta 2)
Calculated molecular weight 212aa,24 kDa
Observed molecular weight
GenBank accession number BC015511
Gene symbol IL6
Gene ID (NCBI) 3569
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

Protocols

Product Specific Protocols
IHC protocol for IL-6 antibody 21865-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Pharmacol

Protective Effects of Costunolide Against D-Galactosamine and Lipopolysaccharide-Induced Acute Liver Injury in Mice.

Authors - Jingxin Mao
mouseWB,IHC

Mediators Inflamm

The Protective Effects of Levo-Tetrahydropalmatine on ConA-Induced Liver Injury Are via TRAF6/JNK Signaling.

Authors - Qiang Yu
mouseWB

Biomed Res Int

Rapamycin and FTY720 Alleviate Atherosclerosis by Cross Talk of Macrophage Polarization and Autophagy.

Authors - Rui-Zhen Sun
mouseWB

Int Immunopharmacol

Hepatoprotective effects and structure-activity relationship of five flavonoids against lipopolysaccharide/d-galactosamine induced acute liver failure in mice.

Authors - Yutong He
humanWB

Invest Ophthalmol Vis Sci

Selective Serotonin Reuptake Inhibitors Aggravate Depression-Associated Dry Eye Via Activating the NF-κB Pathway.

Authors - Xiaozhao Zhang
mouseWB

Osteoarthritis Cartilage

Clock mutant promotes osteoarthritis by inhibiting the acetylation of NFκB.

Authors - G Yuan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Elizabeth Clawson (12-03-2018)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse
No image submitted with review