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- KD/KO Validated
IL-6 Polyclonal antibody
IL-6 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IF
Conjugate
Unconjugated
306
Cat no : 21865-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | LPS and Protein transport inhibitor treated HUVEC cells |
| Positive IHC detected in | human tonsillitis tissue, human spleen tissue, human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 221 publications below |
| IHC | See 79 publications below |
| IF | See 23 publications below |
| ELISA | See 3 publications below |
Product Information
The immunogen of 21865-1-AP is IL-6 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | canine, goat, human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IL-6 fusion protein Ag10565 |
| Full Name | interleukin 6 (interferon, beta 2) |
| Calculated molecular weight | 212 aa, 24 kDa |
| Observed molecular weight | 20 kDa |
| GenBank accession number | BC015511 |
| Gene symbol | IL6 |
| Gene ID (NCBI) | 3569 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-6 antibody 21865-1-AP | Download protocol |
| IHC protocol for IL-6 antibody 21865-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Pharmacol Protective Effects of Costunolide Against D-Galactosamine and Lipopolysaccharide-Induced Acute Liver Injury in Mice. | ||
Mediators Inflamm The Protective Effects of Levo-Tetrahydropalmatine on ConA-Induced Liver Injury Are via TRAF6/JNK Signaling. | ||
Biomed Res Int Rapamycin and FTY720 Alleviate Atherosclerosis by Cross Talk of Macrophage Polarization and Autophagy. | ||
Int Immunopharmacol Hepatoprotective effects and structure-activity relationship of five flavonoids against lipopolysaccharide/d-galactosamine induced acute liver failure in mice. | ||
Invest Ophthalmol Vis Sci Selective Serotonin Reuptake Inhibitors Aggravate Depression-Associated Dry Eye Via Activating the NF-κB Pathway. | ||
Osteoarthritis Cartilage Clock mutant promotes osteoarthritis by inhibiting the acetylation of NFκB. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Elizabeth (Verified Customer) (12-03-2018) |
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