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IL-6 Antibody 73 Publications

Rabbit Polyclonal| Catalog number: 21865-1-AP

Featured Product KD/KO validated

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Con: 53 μg/150 μl

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Tested Applications

Applications:
WB, IHC, ELISA
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IL-6 Antibody WB human plasma tissue 21865-1-AP

human plasma tissue were subjected to SDS PAGE followed by western blot with 21865-1-AP (IL-6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

IL-6 Antibody IHC human tonsillitis tissue 21865-1-AP

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 21865-1-AP (IL-6 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

IL-6 Antibody IHC human tonsillitis tissue 21865-1-AP

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 21865-1-AP (IL-6 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, goat, mouse, rat
Positive WB detected in:
human plasma tissue, Ag13767
Positive IHC detected in:
human tonsillitis tissue, human spleen tissue, human hepatocirrhosis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
IL-6 fusion protein Ag10565
Full name:
interleukin 6 (interferon, beta 2)
Calculated molecular weight:
212aa,24 kDa
Observed molecular weight:
24 kDa
GenBank accession number:
BC015511
Gene ID (NCBI):
3569
Gene symbol
IL6
Synonyms
B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HGF, HSF, Hybridoma growth factor, IFN beta 2, IFNB2, IL 6, IL6, IL-6, Interferon beta 2, Interleukin 6

Background

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.


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