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IL-6R Polyclonal antibody

IL-6R Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 23457-1-AP


CD126, gp80, IL 6 receptor subunit alpha, IL 6R 1, IL 6R alpha, IL 6R subunit alpha, IL 6RA, IL-6 R alpha, IL-6R, IL6RA, interleukin 6 receptor, Membrane glycoprotein 80

Tested Applications

Positive WB detected inJurkat cells, mouse spleen tissue, rat spleen tissue
Positive IP detected inmouse spleen tissue
Positive IHC detected inhuman tonsillitis tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23457-1-AP is IL-6R Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-6R fusion protein Ag18263
Full Name interleukin 6 receptor
Calculated molecular weight 468 aa, 52 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC132684
Gene symbol IL6R
Gene ID (NCBI) 3570
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R, the complex of IL-6 and IL-6R associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while expression of IL-6R is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892). Soluble form of IL-6R has been found in human serum and urine (PMID: 2529343; 1545125).


Product Specific Protocols
WB protocol for IL-6R antibody 23457-1-APDownload protocol
IHC protocol for IL-6R antibody 23457-1-APDownload protocol
IF protocol for IL-6R antibody 23457-1-APDownload protocol
IP protocol for IL-6R antibody 23457-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Allergol Int

Prevention of IL-6 signaling ameliorates toluene diisocyanate-induced steroid-resistant asthma.

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Signal Transduct Target Ther

Targeting polarized phenotype of microglia via IL6/JAK2/STAT3 signaling to reduce NSCLC brain metastasis.

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Kaohsiung J Med Sci

MiR-22 restrains proliferation of rheumatoid arthritis by targeting IL6R and may be concerned with the suppression of NF-κB pathway.

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Anticancer Res

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Microvasc Res

Human cancer cells suppress behaviors of endothelial progenitor cells through miR-21 targeting IL6R.

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