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IL-6R alpha Polyclonal antibody

IL-6R alpha Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 23457-1-AP


CD126, gp80, IL 6 receptor subunit alpha, IL 6R 1, IL 6R alpha, IL 6R subunit alpha, IL 6RA, IL-6 R alpha, IL-6R, IL-6R alpha, IL6RA, interleukin 6 receptor, Membrane glycoprotein 80

Tested Applications

Positive WB detected inJurkat cells, mouse spleen tissue, rat spleen tissue
Positive IP detected inmouse spleen tissue

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

23457-1-AP targets IL-6R alpha in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-6R alpha fusion protein Ag18263
Full Name interleukin 6 receptor
Calculated molecular weight 468 aa, 52 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC132684
Gene symbol IL6R
Gene ID (NCBI) 3570
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R alpha, the complex of IL-6 and IL-6R alpha associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while expression of IL-6R alpha is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892). Soluble form of IL-6R alpha has been found in human serum and urine (PMID: 2529343; 1545125).


Product Specific Protocols
WB protocol for IL-6R alpha antibody 23457-1-APDownload protocol
IP protocol for IL-6R alpha antibody 23457-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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