|Positive WB detected in||HEK-293 cells, MCF-7 cells, HT-1080 cells, HeLa cells|
|Positive IP detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Sample-dependent, check data in validation data gallery|
The immunogen of 20433-1-AP is INSR Fusion Protein expressed in E. coli.
|Cited Reactivity||fish, human, mouse, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||INSR fusion protein Ag13922|
|Calculated molecular weight||1382 aa, 156 kDa|
|Observed molecular weight||190 kDa, 135 kDa, 95 kDa|
|GenBank accession number||BC117172|
|Gene ID (NCBI)||3643|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The biological effects of INS are mediated by a membrane spanning cell surface receptor that has been shown to consist of disulfide-linked 135-kDa and 95-kDa alpha- and beta-subunits that are cleaved products of a common precursor. Binding of INS to the receptor extracellular domain results in intracellular activation of a tyrosine-specific kinase activity and the generation of signals that determine the cellular response (PMID: 2369896). This antibody raised against 980-1382aa of human INS receptor can recognize full-length INS receptor precursor and INS receptor beta subunit.
Cell Physiol Biochem
Acid Loading Unmasks Glucose Homeostatic Instability in Proximal-Tubule-Targeted Insulin/Insulin-Like-Growth-Factor-1 Receptor Dual Knockout Mice.
Comp Biochem Physiol A Mol Integr Physiol
Inhibited autophagy impairs systemic nutrient metabolism in Nile tilapia.
Fish Physiol Biochem
The reduction of lipid-sourced energy production caused by ATGL inhibition cannot be compensated by activation of HSL, autophagy, and utilization of other nutrients in fish.
Postnatal overfeeding induces hepatic microRNA-221 expression and impairs the PI3K/AKT pathway in adult male rats.
Toxicol Appl Pharmacol
RNA-binding protein CUGBP1 controls the differential INSR splicing in molecular subtypes of breast cancer cells and affects cell aggressiveness.