|Positive WB detected in||HEK-293 cells, MCF-7 cells, HT-1080 cells, HeLa cells|
|Positive IP detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Sample-dependent, check data in validation data gallery|
The immunogen of 20433-1-AP is INSR Fusion Protein expressed in E. coli.
|Cited Reactivity||fish, human, mouse, rat, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||INSR fusion protein Ag13922|
|Calculated molecular weight||1382 aa, 156 kDa|
|Observed molecular weight||190 kDa, 135 kDa, 95 kDa|
|GenBank accession number||BC117172|
|Gene ID (NCBI)||3643|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The biological effects of INS are mediated by a membrane spanning cell surface receptor that has been shown to consist of disulfide-linked 135-kDa and 95-kDa alpha- and beta-subunits that are cleaved products of a common precursor. Binding of INS to the receptor extracellular domain results in intracellular activation of a tyrosine-specific kinase activity and the generation of signals that determine the cellular response (PMID: 2369896). This antibody raised against 980-1382aa of human INS receptor can recognize full-length INS receptor precursor and INS receptor beta subunit.
Cell Physiol Biochem
Acid Loading Unmasks Glucose Homeostatic Instability in Proximal-Tubule-Targeted Insulin/Insulin-Like-Growth-Factor-1 Receptor Dual Knockout Mice.
Comp Biochem Physiol A Mol Integr Physiol
Inhibited autophagy impairs systemic nutrient metabolism in Nile tilapia.
Fish Physiol Biochem
The reduction of lipid-sourced energy production caused by ATGL inhibition cannot be compensated by activation of HSL, autophagy, and utilization of other nutrients in fish.
Postnatal overfeeding induces hepatic microRNA-221 expression and impairs the PI3K/AKT pathway in adult male rats.
Toxicol Appl Pharmacol
RNA-binding protein CUGBP1 controls the differential INSR splicing in molecular subtypes of breast cancer cells and affects cell aggressiveness.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Boyan (Verified Customer) (06-06-2021)
This antibody recognized a band at ~ 180 kd only, which is sensitive to PNGaseF, but not EndoH, treatment. The size is much bigger than an expected InsR band. Importantly, stripping the membrane and re-blot with another InsR antibody from a different brand could detect the protein at the expected MW. These results suggest this InsR antibody is detecting something other than InsR.