INSR Polyclonal antibody

INSR Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IP, IHC, Dot blot,ELISA

Conjugate

Unconjugated

Cat no : 20433-1-AP

Synonyms

CD220, HHF5, INSR, IR



Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells, HT-1080 cells, HeLa cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20433-1-AP is INSR Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity fish, human, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INSR fusion protein Ag13922
Full Name INSR
Calculated molecular weight 1382 aa, 156 kDa
Observed molecular weight 190 kDa, 135 kDa, 95 kDa
GenBank accession numberBC117172
Gene symbol INSR
Gene ID (NCBI) 3643
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The biological effects of INS are mediated by a membrane spanning cell surface receptor that has been shown to consist of disulfide-linked 135-kDa and 95-kDa alpha- and beta-subunits that are cleaved products of a common precursor. Binding of INS to the receptor extracellular domain results in intracellular activation of a tyrosine-specific kinase activity and the generation of signals that determine the cellular response (PMID: 2369896). This antibody raised against 980-1382aa of human INS receptor can recognize full-length INS receptor precursor and INS receptor beta subunit.

Protocols

Product Specific Protocols
WB protocol for INSR antibody 20433-1-APDownload protocol
IP protocol for INSR antibody 20433-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Physiol Biochem

Acid Loading Unmasks Glucose Homeostatic Instability in Proximal-Tubule-Targeted Insulin/Insulin-Like-Growth-Factor-1 Receptor Dual Knockout Mice.

Authors - Abdullah Aljaylani
fishWB

Comp Biochem Physiol A Mol Integr Physiol

Inhibited autophagy impairs systemic nutrient metabolism in Nile tilapia.

Authors - Si-Lan Han
zebrafishWB

Fish Physiol Biochem

The reduction of lipid-sourced energy production caused by ATGL inhibition cannot be compensated by activation of HSL, autophagy, and utilization of other nutrients in fish.

Authors - Si-Lan Han
ratWB

Pediatr Res

Postnatal overfeeding induces hepatic microRNA-221 expression and impairs the PI3K/AKT pathway in adult male rats.

Authors - Fang Huang
ratWB

Toxicol Appl Pharmacol

PMID: 28025108

Authors - Wang Zhang
humanWB

Carcinogenesis

RNA-binding protein CUGBP1 controls the differential INSR splicing in molecular subtypes of breast cancer cells and affects cell aggressiveness.

Authors - Gena Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (06-06-2021)

This antibody recognized a band at ~ 180 kd only, which is sensitive to PNGaseF, but not EndoH, treatment. The size is much bigger than an expected InsR band. Importantly, stripping the membrane and re-blot with another InsR antibody from a different brand could detect the protein at the expected MW. These results suggest this InsR antibody is detecting something other than InsR.

  • Applications: Western Blot