INVS Recombinant antibody, PBS Only (Detector)

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  WB analysis using 85768-2-RR (same clone as 85768-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85768-2-RR (INVS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85768-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02076-1

  Cytometric bead array standard curve of MP02076-1, INVS Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85768-3-PBS. Detection antibody: 85768-2-PBS. Standard: Ag0934. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP02076-2

  Cytometric bead array standard curve of MP02076-2, INVS Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85768-1-PBS. Detection antibody: 85768-2-PBS. Standard: Ag0934. Range: 0.781-100 ng/mL.

INVS Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 85768-2-PBS

Clone No.250038E2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

INV, Inversin, KIAA0573, Nephrocystin 2, Nephrocystin-2

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Product Information

85768-2-PBS targets INVS as part of a matched antibody pair:

MP02076-1: 85768-3-PBS capture and 85768-2-PBS detection (validated in Cytometric bead array)

MP02076-2: 85768-1-PBS capture and 85768-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: INVS fusion protein Ag0934
• Full Name: inversin
• Calculated Molecular Weight: 118 kDa, 100 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC006370
• Gene Symbol: INVS
• Gene ID (NCBI): 27130
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y283
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

INVS, short for inversin, plays a role in primary cilia function and involvement in the cell cycle. It is required for normal renal development and establishment of the left-right axis. INVS may be involved in Wnt signaling pathways by acting as a molecular switch between different them. Inhibits the canonical Wnt pathway by targeting cytoplasmic disheveled (DVL1) for degradation by the ubiquitin-proteasome. This suggests that it is required in renal development to oppose the repression of terminal differentiation of tubular epithelial cells by Wnt signaling.